SNAP-25 Antibody

SNAP-25 Antibody gallery image 1

Western blotting analysis of human cerebellum) (A) and mouse brain (B) lysates (35μg protein in RIPA buffer) probed using Goat anti Human SNAP25 antibody (AHP1124) at 0.03μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

SNAP-25 Antibody gallery image 2

EB06738 (1µg/ml) Immunohistochemical staining of formalin fixed, paraffin embedded Human Cerebellum following heat mediated antigen retrieval in citrate buffer at pH6.0. A. Cerebellum stained using Goat anti Human SNAP25 antibody (AHP1124) at 1.0μgml-1. B. Negative control showing cerebellum staining with primary antibody omitted

  • SNAP-25 Antibody thumbnail image 1
  • SNAP-25 Antibody thumbnail image 2
  • Goat anti SNAP-25 (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1124E, P*, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat ati Human SNAP25 antibody recognizes human synaptosomal-associated protein, 25kD (SNAP-25), also known as Super protein (Sup) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid, presynaptic plasma membrane protein containning 2 tSNARE coiled-coil domains and is essential for Ca2+-regulated exocytosis of neurotransmitters. SNAP-25 binds to and negatively modulate Ca2+ channels, increasing the speed of neurotransmitter release (Rettiq et al. 1996) .Goat ati Human SNAP25 antibody detects an epitope within the C-terminal (CT) of human SNAP25 (aa 193-206).

      As a result of alternative splicing, SNAP-25 is expressed by neurons and neuroendocrine cells as two predominant isoforms; SNAP-25a and SNAP-25b. SNAP-25a is expressed early in development, whereas SNAP-25b expression is linked to synaptogenesis (Bark and Wilson 1994). Goat ati human SNAP25 antibody is expected to recognise both isoforms.

      SNAP-25 may be a possible therapeutic treatment target for sufferers of hyperactivity disorders (Barr et al. 2000), whilst SNAP-25 based peptides may be a possible treatment for involuntary muscle spasms (Hodel 1998).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      DogExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human SNAP-25 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence C-DEANQRATKMLGSG corresponding to the C-terminus of SNAP-25 (NP_003072.2, NP_570824.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.010.03ug/ml
      Immunohistology - Paraffin(1)2.04.0ug/ml
      Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Bio-Rad recommend citrate buffer, pH6.0, for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1124 detects a band of approximately 22kDa in human and mouse brain lysates. A minimum incubation time of 1 hour is recommended with this antibody.
    • Instructions For Use

    Additional SNAP-25 Antibody Formats

    Formats Applications Sizes available
    SNAP-25 Antibody : Purified E, P*, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls


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