IL-6 Antibody

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IL-6 Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human cervical squamous cell carcinoma with Rabbit anti Human interleukin-6 (AHP1040)

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IL-6 Antibody gallery image 2

Immunofluorescent staining of BJ human foreskin fibroblast cells with Rabbit anti Human interleukin-6 (AHP1040), green; counterstained with tubulin, red and nuclei, blue

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IL-6 Antibody gallery image 3

Immunofluorescent staining of HeLa cells with Rabbit anti Human interleukin-6 (AHP1040), green; counterstained with tubulin, red and nuclei, blue

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IL-6 Antibody gallery image 4

Immunofluorescent staining of PC3, prostate cancer cells with Rabbit anti Human interleukin-6 (AHP1040), green; counterstained with tubulin, red and nuclei, blue

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  • IL-6 Antibody thumbnail image 1
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  • Rabbit anti Human Interleukin-6
  • Rabbit anti Human Interleukin-6:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1040E, FN*, IF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1040
    AHP1040BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP1040B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human Interleukin-6 antibody is specific for human IL-6 (Interleukin-6), a 184 amino acid multifunctional pro-inflammatory cytokine, secreted by activated T cells, macrophages, endothelial cells, fibroblasts and osteoblasts.

      The diverse biological properties of IL-6 include the principle inducer of the acute phase response in the liver, stimulation of B cell differentiation and subsequent immunoglobulin production, promotion of hybridoma growth, regulation of bone metabolism and stimulation of energy mobilisation in muscle and fatty tissue. IL-6 signals through a membrane bound receptor complex consisting of the ligand-binding IL-6Rα chain (CD126) and the signal-transducing component gp130 (CD130), but can also stimulate cells expressing gp130 through a soluble IL-6R.

      Rabbit anti Human Interleukin-6 antibody has been shown to inhibit the biological activity of Human IL-6.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml
      Immunofluorescence
      Western Blotting0.10.2ug/ml
      Functional Assays(1)0.200.40ug/ml
      Immunohistology - Paraffin(2)0.250.5ug/ml
      (1)
      To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-6 (1.0 ng/ml).
      (2)
      This product requires heat-mediated antigen retrieval prior to staining paraffin-embedded sections. Citrate buffer, pH6.0, is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.150.30ug/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Purified rabbit anti human IL-6 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated rabbit anti human IL-6 (AHP1040B) as the detection reagent and recombinant human IL-6 (PHP045) as the standard.
    • ELISA
      This biotinylated rabbit anti human IL-6 may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IL-6 antibody (AHP1040) as the capture reagent and recombinant human IL-6 (PHP045) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      Rabbit anti human IL-6 may used under either reducing or non-reducing conditions with recombinant human IL-6 (PHP045) as the positive control
    • Western Blotting
      Rabbit anti human IL-6 may used under either reducing or non-reducing conditions with recombinant human IL-6 (PHP045) as the positive control
    • Instructions For Use
    • Instructions For Use

    Additional IL-6 Antibody Formats

    Formats Applications Sizes available
    IL-6 Antibody : Biotin E, WB 50 µg
    IL-6 Antibody : Purified E, FN*, IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Rabbit anti Human Interleukin-6:BiotinAHP1040B50 µgE, WB
          AHP1040B
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interleukin-6AHP10400.1 mgE, FN*, IF, P *, WB
          AHP1040

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Interleukin-6PHP04520 µgE, FN, WB
            PHP045
            Recombinant Human Interleukin-6PHP045A5 µgE, FN, WB
            PHP045A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Interleukin-6PHP04520 µgE, FN, WB
            PHP045
            Recombinant Human Interleukin-6PHP045A5 µgE, FN, WB
            PHP045A

            Histology Controls

              Further Reading

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