IFN Gamma Antibody | 2503

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IFN Gamma Antibody | 2503 gallery image 1

Graph showing detection of human interferon gamma using a combination of HCA043 and HCA044

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  • IFN Gamma Antibody | 2503 thumbnail image 1
  • Human anti Human Interferon Gamma
  • Human anti Human Interferon Gamma
  • Human anti Human Interferon Gamma:HRP
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  • Product Type
    Monoclonal Antibody
  • Clone
    2503
  • Isotype
    HuCAL Fab bivalent
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA044E *datasheet pdfdatasheet pdf0.1 mg
    HCA044
    HCA044PE *datasheet pdfdatasheet pdf0.1 mg
    HCA044P
    HCA044AE *datasheet pdfdatasheet pdf50 µg
    HCA044A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human Interferon gamma antibody, clone 2503 recognizes human IFN gamma, a key cytokine in the immune response.
    • Intended Use
    • Target Species
      Human
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS tag at the C-terminus of the antibody heavy chain.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS tag at the C-terminus of the antibody heavy chain.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid, conjugated to Horseradish Peroxidase (HRP).
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      None present.
      None present.
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Immunogen
      Purified recombinant human interferon gamma
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.1mg/ml following reconstitution
      Antibody concentration 0.1mg/ml following reconstitution
      Protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of reconstitution.
      6 months from date of reconstitution.
      12 months from date of despatch.
    • GO Terms
      positive regulation of peptidyl-serine phosphorylation of STAT protein
      negative regulation of gene-specific transcription from RNA polymerase II promoter
      protein import into nucleus, translocation
      positive regulation of interleukin-23 production
      cell cycle arrest
      positive regulation of tyrosine phosphorylation of Stat1 protein
      positive regulation of mesenchymal cell proliferation
      positive regulation of interleukin-12 production
      positive regulation of osteoclast differentiation
      negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
      response to virus
      positive regulation of fructose 1,6-bisphosphate metabolic process
      positive regulation of nitric oxide biosynthetic process
      negative regulation of smooth muscle cell proliferation
      regulation of interferon-gamma-mediated signaling pathway
      positive regulation of killing of cells of other organism
      cytokine activity
      positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
      immune response
      positive regulation of vitamin D biosynthetic process
      tyrosine phosphorylation of Stat1 protein
      extracellular space
      positive regulation of calcidiol 1-monooxygenase activity
      positive regulation of membrane protein ectodomain proteolysis
      negative regulation of metanephric nephron tubule epithelial cell differentiation
      interferon-gamma-mediated signaling pathway
      positive regulation of smooth muscle cell apoptosis
      interferon-gamma receptor binding
      regulation of insulin secretion
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA(1)1ug/ml
      (1)
      For use as a capture reagent in Sandwich ELISA we recommend the use of HISPEC ASSAY DILUENT for sample and antibody dilution.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA(1)1ug/ml
      (1)
      For use as a capture reagent in Sandwich ELISA we recommend the use of HISPEC ASSAY DILUENT for sample and antibody dilution.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA(1)1ug/ml
      (1)
      For use as a capture reagent in Sandwich ELISA we recommend the use of HISPEC ASSAY DILUENT for sample and antibody dilution.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      HCA044A may be used in a sandwich ELISA assay as detection antibody in combination with HCA043 as capture antibody.
    • ELISA
      HCA044 may be used in a sandwich ELISA assay as detection antibody in combination with HCA043 as capture antibody.
    • ELISA
      HCA044P may be used in a sandwich ELISA assay as detection antibody in combination with HCA043 as capture antibody.
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Clone Applications Sizes available
    IFN Gamma Antibody : Purified 2503 E * 0.1 mg | 50 µg
    IFN Gamma Antibody : HRP 2503 E * 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
      MCA4764P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
      MCA4764P
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

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