IFN Gamma Antibody | 0676

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Sandwich ELISA showing detection of human interferon gamma using HCA043 as a capture reagent and HCA044 as a detection reagent

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  • Human anti Human Interferon Gamma
  • Human anti Human Interferon Gamma
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    0676
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA043E *datasheet pdfdatasheet pdf0.1 mg
    HCA043
    HCA043AE *datasheet pdfdatasheet pdf50 µg
    HCA043A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human Interferon gamma antibody, clone 0676 recognizes human IFN gamma, a key cytokine in the immune response.
    • Intended Use
    • Target Species
      Human
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
    • Reconstitution
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.01% Thiomersal
      0.01% Thiomersal
    • Immunogen
      Purified recombinant human interferon gamma
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.5mg/ml
      Antibody concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      6 months from date of despatch.
      6 months from date of despatch.
    • GO Terms
      tyrosine phosphorylation of Stat1 protein
      positive regulation of osteoclast differentiation
      positive regulation of mesenchymal cell proliferation
      positive regulation of membrane protein ectodomain proteolysis
      interferon-gamma receptor binding
      positive regulation of tyrosine phosphorylation of Stat1 protein
      positive regulation of peptidyl-serine phosphorylation of STAT protein
      negative regulation of smooth muscle cell proliferation
      positive regulation of calcidiol 1-monooxygenase activity
      positive regulation of interleukin-12 production
      positive regulation of smooth muscle cell apoptosis
      cytokine activity
      negative regulation of gene-specific transcription from RNA polymerase II promoter
      positive regulation of nitric oxide biosynthetic process
      positive regulation of fructose 1,6-bisphosphate metabolic process
      negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
      interferon-gamma-mediated signaling pathway
      positive regulation of killing of cells of other organism
      positive regulation of vitamin D biosynthetic process
      response to virus
      immune response
      regulation of interferon-gamma-mediated signaling pathway
      regulation of insulin secretion
      positive regulation of interleukin-23 production
      extracellular space
      cell cycle arrest
      negative regulation of metanephric nephron tubule epithelial cell differentiation
      positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
      protein import into nucleus, translocation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA(1)1ug/ml
      (1)
      For use as a capture reagent in Sandwich ELISA we recommend the use of HISPEC ASSAY DILUENT for sample and antibody dilution.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA(1)1ug/ml
      (1)
      For use as a capture reagent in Sandwich ELISA we recommend the use of HISPEC ASSAY DILUENT for sample and antibody dilution.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      HCA043A may be used in a sandwich ELISA assay as a capture antibody in combination with (HCA044) as detection antibody.
    • ELISA
      HCA043 may be used in a sandwich ELISA assay as a capture antibody in combination with (HCA044) as detection antibody.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Clone Applications Sizes available
    IFN Gamma Antibody : Purified 0676 E * 50 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hispec Assay DiluentBUF049A50 mlIY
          BUF049A

          Recommended Positive Controls

            Histology Controls

              References

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