HLA ABC antibody | YTH862.2
Rat anti Human HLA ABC antibody, clone YTH862.2 (MCA485G) used to evaluate MHC class I expression in myotubes by immunofluorescence.
Image caption:
Stimulation with IL-1β and IFN-γ of muscle cells (myotubes) leads to an increased expression of MHC-I and NLRP3. (A) Immunfluorescence images of muscle cells without and with stimulation with IL-1β (20 ng/mL) and IFN-γ (300 U/mL) for 24 h, 48 h and 72 h, respectively. Cells were fixed at indicated times post treatment and immunostained for MHC-I (green) and NLRP3 (red). Image acquisition was performed using an inverted transmitted light microscope. Scale bar corresponds to 40 μm.
From: Kummer K, Bertram I, Zechel S, Hoffmann DB, Schmidt J.
Inflammasome in Skeletal Muscle: NLRP3 Is an Inflammatory Cell Stress Component in Inclusion Body Myositis.
Int J Mol Sci. 2023 Jun 26;24(13):10675.
doi: 10.3390/ijms241310675.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Human HLA ABC
- Product Type
- Monoclonal Antibody
- Clone
- YTH862.2
- Isotype
- IgG2b
- Specificity
- HLA ABC
Antibody Quality and Performance Guarantee
| Rat anti Human HLA ABC antibody, clone YTH862.2 recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells. Rat anti Human HLA ABC antibody, clone YTH862.2 may be able to induce apoptosis in activated but not resting T cells (Genestier et al. 1997). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- PHA activated human peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized DA rats were fused with cells of the rat Y3 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Functional Assays 1 | |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2b Negative Control | MCA6006GA | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
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Log in | ||||||
| Description | Rat IgG2b Negative Control | ||||||
References for HLA ABC antibody
-
Schmidt, C.M. et al. (1997) Cytotoxic T lymphocyte recognition of HLA-G in mice.
Hum Immunol. 55 (2): 127-39. -
Genestier, L. et al. (1997) Antibodies to HLA class I alpha1 domain trigger apoptosis of CD40-activated human B lymphocytes.
Blood. 90 (2): 726-35. -
Genestier, L. et al. (1997) Fas-independent apoptosis of activated T cells induced by antibodies to the HLA class I alpha1 domain.
Blood. 90 (9): 3629-39. -
Genestier, L. et al. (1998) Caspase-dependent ceramide production in Fas- and HLA class I-mediated peripheral T cell apoptosis.
J Biol Chem. 273 (9): 5060-6. -
Tanaka, J. et al. (2004) Cytolytic activity and regulatory functions of inhibitory NK cell receptor-expressing T cells expanded from granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells.
Blood. 104 (3): 768-74. -
Schmidt, J. et al. (2004) Upregulated inducible co-stimulator (ICOS) and ICOS-ligand in inclusion body myositis muscle: significance for CD8+ T cell cytotoxicity.
Brain. 127: 1182-90. -
Liu, S. et al. (2005) Cross-talk between Schwann cells and neuroblasts influences the biology of neuroblastoma xenografts.
Am J Pathol. 166 (3): 891-900. -
Schmidt, J. et al. (2008) Interrelation of inflammation and APP in sIBM: IL-1 beta induces accumulation of beta-amyloid in skeletal muscle.
Brain. 131 (Pt 5): 1228-40.
View The Latest Product References
-
Muth, I.E. et al. (2009) Proinflammatory cell stress in sporadic inclusion body myositis muscle: overexpression of alphaB-crystallin is associated with amyloid precursor protein and accumulation of beta-amyloid.
J Neurol Neurosurg Psychiatry. 80: 1344-9. -
Muth, I.E. et al. (2015) HMGB1 and RAGE in skeletal muscle inflammation: Implications for protein accumulation in inclusion body myositis.
Exp Neurol. 271: 189-97. -
Limozin, L. et al. (2016) A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.
Sci Rep. 6: 35193. -
Schmidt, K. et al. (2016) Molecular treatment effects of alemtuzumab in skeletal muscles of patients with IBM.
BMC Neurol. 16 (1): 48. -
Olivero, C. et al. (2019) Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells.
Methods Mol Biol. 1879: 415-33. -
Kummer, K. et al. (2023) Inflammasome in Skeletal Muscle: NLRP3 Is an Inflammatory Cell Stress Component in Inclusion Body Myositis.
Int J Mol Sci. 24 (13): 10675.
- RRID
- AB_567133
MCA485G
158669
1804
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