CD8 Antibody | YTC182.20

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CD8 Antibody | YTC182.20 gallery image 1

Staining of human peripheral blood lymphocytes with Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G)

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Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the endocervix of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8+ T cells in cervix and vagina.
A) Top panels show representative images of endocervix from an animal (137.09) at 5 weeks post-SIV-Δnef vaccination as examples of the low end of the spectrum of levels of tetramer+ detected in genital tissues amongst the whole cohort. Mamu-A1/Gag tetramer+ cells are red to pink, CD8+ T cells are blue, and CD20+ cells are green. B) In the bottom panels Mamu-A1/Tat MHC-tetramer-binding cells are red to pink, CD3+ T cells are blue, and CD20+ B cells are green from a representative image of vagina from animal #338.02, 21 weeks post-SIV-Δnef vaccination and 7 days following vaginal challenge with WT-SIV, as an example of the higher end of the spectrum of levels of tetramer+ cells detected in genital tissues amongst the whole cohort. The edge of the epithelium (E) is indicated with a white line; lamina propria indicated by LP. For both A) and B), the left panel shows a montage of several confocal projected z-series images (projected z-series are images collected a different depths stacked on top of each other), collected with a 20× objective. The right panels shows enlargements with several red to pink tetramer+ cells evident (indicated by arrows in A) localized diffusely as well as within small T cell aggregates. Scale bars = 100μm.

From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.

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CD8 Antibody | YTC182.20 gallery image 3

Staining of human peripheral blood lymphocytes with Rat anti Human CD8:FITC antibody, clone YTC182.20 (MCA351F)

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CD8 Antibody | YTC182.20 gallery image 4

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the vagina of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8 T cells in vagina at 14 days post-vaccination.
Representative confocal image showing Mamu-A1/Gag tetramer+ cells (red), CD8 (blue), and CD20 (green). The right panel shows a montage of projected z-series of several 200× fields with the epithelium (E), lamina propria (LP), muscularis (M), and areas with vessels (V) indicated. In the enlarged image on the left, tetramer+ cells are indicated with arrows. Scale bars are 100μm.

From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.

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CD8 Antibody | YTC182.20 gallery image 5

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the endocervix of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Most CD3+ T cells were CD8+ in genital lymphoid aggregates.
Representative images of endocervix section stained with Mamu-A1/Gag tetramers (red), CD8 antibodies (green), and CD3 antibodies (blue) from animal #338.02. Tetramer+ cells are indicated with white arrows in the enlargement. The bottom panels show individual tetramer and antibody stains. Note most CD3+ T cells are CD8+. The top left panel shows a montage of projected z-series of several 200× fields. Scale bars = 100μm.

From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.

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CD8 Antibody | YTC182.20 gallery image 6

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the lymphoid organs of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8+ T cells in lymph nodes and spleen.
The representative images shown in are A) from an axillary lymph node from an animal (119.96) 5 weeks post-SIV-Δnef vaccination. Mamu-A1/Tat tetramer+ cells are red to pink, CD8+ T cells are green, and CD3+ T cells are blue. The left panel shows a montage of several fields containing projected z-scans collected with a confocal microscope 20× objective. Enlargements to the right show individual fields of projected z-scans collected with a 60× objective.
red pulp (RP) marginal zones (MZ) T cell zones (T) B cell follicles (B).

From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.

Enlarge
CD8 Antibody | YTC182.20 gallery image 7

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the lymphoid organs of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8+ T cells in lymph nodes and spleen.
The representative images shown in B) are from a spleen section from an animal (119.96) at 5 weeks post-SIVΔnef vaccination. Mamu-A1/Gag tetramer+ cells are red to pink colored, CD8+ T cells are blue, and CD20+ B cells are green. Tetramer+CD8+ cells localized with other CD8 T cells and were most abundant in the red pulp (RP) and marginal zones (MZ), with relatively fewer located in T cell zones (T) and in B cell follicles (B). The left panel shows a montage of several confocal microscope fields of projected Z-scans stitched together, collected with a 10× objective. Enlargements show individual confocal z-scans of single 200× fields.

From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.

Enlarge
CD8 Antibody | YTC182.20 gallery image 8

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used to identify CD8+ expressing cells in the Peyer's patches of Rhesus macaques (Macaca mulatta) by immunofluorescence.
Image caption:
Localization of SIV-specific CD8 T cells in ileal Peyer's patches.
The representative images shown are from animal #174.96 stained with Mamu-A1/gag tetramers (red), CD20 antibodies (green), and CD3 antibodies (blue). Tetramer+ cells were localized throughout the Peyer's patches of ileum tissue sections with other T cells around as well as within B cell follicles. The left shows a montage of several confocal Z-scan fields stitched together, collected with a 20× objective.

From: Sasikala-Appukuttan AK, Kim HO, Kinzel NJ, Hong JJ,
Smith AJ, Wagstaff R, et al. (2013)
Location and Dynamics of the Immunodominant CD8 T Cell Response to SIVΔnef Immunization and SIVmac251 Vaginal Challenge.
PLoS ONE 8(12): e81623.

Enlarge
CD8 Antibody | YTC182.20 gallery image 9

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
Representative maximum intensity projections of z-stack laser confocal microscopy images of immunofluorescent triple stainings for grB (green color), CD8 (red color) and the early apoptotic cell marker “cleaved caspase-3” (cCASP3; white color). Stained sections were counterstained with DAPI (blue color). Representative stainings of three mAIL are shown of 12 immunohistochemically classified WML tissues of 10 MS patients analyzed. Punctated (inset 1) and polarized grB expression by CD8+ T cells (insets 3 and 5), as well as grB-negative CD8+ T cells are shown (insets 2, 4 and 6). Co-localization of grB and cCASP3 is observed in a cell adjacent to a CD8+ T-cell with polarized grB suggesting grB-mediated killing of the respective target cell (inset 5). Dotted line represents the glia limitans separating the perivascular space and parenchyma.

From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 2017 Jun 17. [Epub ahead of print]

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CD8 Antibody | YTC182.20 gallery image 10

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
CD8+ T cells interact with all major brain-resident cell types in white matter lesions of MS patients. Representative double-immunofluorescence stainings on 8-μm sections of 12 formalin-fixed paraffin-embedded white matter lesion (WML) tissues from 10 MS patients are shown for: glial fibrillary acidic protein (GFAP: marker for astrocytes - shown green).

From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 2017 Jun 17. [Epub ahead of print]

Enlarge
CD8 Antibody | YTC182.20 gallery image 11

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
CD8+ T cells interact with all major brain-resident cell types in white matter lesions of MS patients. Representative double-immunofluorescence stainings on 8-μm sections of 12 formalin-fixed paraffin-embedded white matter lesion (WML) tissues from 10 MS patients are shown for: ionized calcium-binding adapter molecule 1 (Iba1: marker for microglia - shown green).

From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 2017 Jun 17. [Epub ahead of print]

Enlarge
CD8 Antibody | YTC182.20 gallery image 12

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
CD8+ T cells interact with all major brain-resident cell types in white matter lesions of MS patients. Representative double-immunofluorescence stainings on 8-μm sections of 12 formalin-fixed paraffin-embedded white matter lesion (WML) tissues from 10 MS patients are shown for: proteolipid protein (MCA839G) (PLP: marker for oligodendrocytes - shown green)

From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 2017 Jun 17. [Epub ahead of print]

Enlarge
CD8 Antibody | YTC182.20 gallery image 13

Published customer image:
Rat anti Human CD8 antibody, clone YTC182.20 (MCA351G) used for evaluation of CD8 localization in multiple sclerotic brain sections by immunofluorescence.
Image caption:
CD8+ T cells interact with all major brain-resident cell types in white matter lesions of MS patients. Representative double-immunofluorescence stainings on 8-μm sections of 12 formalin-fixed paraffin-embedded white matter lesion (WML) tissues from 10 MS patients are shown for: neurofilament heavy chain (NF-H: marker for neurons - shown green)

From: van Nierop GP, van Luijn MM, Michels SS, Melief MJ, Janssen M, Langerak AW, Ouwendijk WJD, Hintzen RQ, Verjans GMGM.
Phenotypic and functional characterization of T cells in white matter lesions of multiple sclerosis patients.
Acta Neuropathol. 2017 Jun 17. [Epub ahead of print]

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  • Rat anti Human CD8
  • Rat anti Human CD8
  • Rat anti Human CD8:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YTC182.20
  • Isotype
    IgG2b
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA351GC, Fdatasheet pdfdatasheet pdf0.2 mg
    MCA351G
    MCA351FFdatasheet pdfdatasheet pdf100 Tests
    MCA351F
    MCA351GTC, Fdatasheet pdfdatasheet pdf25 µg
    MCA351GT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a ~30/32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Cynomolgus monkeyyes
      Rhesus Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Mouse L cells transfected with human CD8 gene.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 1.0mg/ml
      Ig concentration 1.0mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      antigen processing and presentation
      regulation of immune response
      integral to plasma membrane
      transmembrane receptor protein tyrosine kinase signaling pathway
      coreceptor activity
      T cell receptor complex
      extracellular region
      MHC class I protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      The isolation and characterisation of this cell line was carried out with the help of funds supplied by the Medical Research Council, United kingdom.
      The isolation and characterisation of this cell line was carried out with the help of funds supplied by the Medical Research Council, United kingdom.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Antibody : Purified YTC182.20 C, F 0.2 mg | 25 µg
    CD8 Antibody : FITC YTC182.20 F 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:FITCMCA6006F0.1 mgF
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
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          Human SeroblockBUF070A50 TestF
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          Recommended Positive Controls

            Histology Controls

              References

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