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CD55 Antibody | Bu14
Staining of human peripheral blood monocytes with Mouse anti Human CD55 (MCA2659)
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Staining of human peripheral blood monocytes with Mouse anti Human CD55:FITC (MCA2659F)
Enlarge- Mouse anti Human CD55
- Mouse anti Human CD55:FITC
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Product TypeMonoclonal Antibody
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CloneBu14
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IsotypeIgG1
2 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Human CD55 antibody, clone Bu14 recognizes human CD55, also known as Decay Accelerating Factor (DAF). CD55 is a glycosylphosphatylinositol (GPI) anchored type I surface protein which prevents complement mediated lysis of autologous cells. CD55 functions to limit the formation and accelerate the decay of C3/C5-convertases from the classic and alternative pathway.
The molecular weight of CD55 varies between ~50 and 100 kDa dependent on cell type. Alternative transcripts can lead to soluble DAF, which is detectable in plasma, tears, saliva, urine and cerebrospinal fluid (Medof et al. 1987).
CD55 acts as a ligand for CD97 (Hamaan et al. 1998) and is an entry receptor for Echovirus (Bergelson et al. 1994) and Coxsackie B virus (Hafenstein et al. 2007). CD55 has been linked to many diseases including multiple sclerosis, rheumatoid arthritis and cancer development (Visser et al. 2002).
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Intended Use
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Target SpeciesHuman
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Product FormPurified IgG - liquidPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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Preservative Stabilisers
0.09% Sodium Azide (NaN3) 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin -
Purity
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Approx. Protein ConcentrationsIgG concentration 1.0mg/mlIgG concentration 0.1 mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered saline
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.18 months from date of despatch.
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GO Termssoluble fractionrespiratory burstelevation of cytosolic calcium ion concentrationanchored to membraneextracellular regioninnate immune responsemembrane raftintegral to plasma membranecomplement activation, classical pathway
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat 1/200 Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat 1/10 - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- Flow CytometryUse 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Instructions For Use
- Instructions For Use
Additional CD55 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| CD55 Antibody : FITC | Bu14 | F | 0.1 mg |
| CD55 Antibody : Purified | Bu14 | F | 0.2 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | MCA928 |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | MCA928F |
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070B | 200 Test | F | BUF070B | |
| Human Seroblock | BUF070A | 50 Test | F | BUF070A |
Recommended Positive Controls
Histology Controls
References
1. Loveland, B.E. and Szokolai, K. (1998) CD55 Workshop Report NL11In: Leukocyte Typing VI, Eds Kishmoto, T. et al. Garland publishing . 519-520.
Further Reading
1. Ruiz-Argüelles, A. & Llorente, L. (2007) The role of complement regulatory proteins (CD55 and CD59) in the pathogenesis of autoimmune hemocytopenias.Autoimmun Rev. 6 (3): 155-61.
2. Mikesch, J.H. et al. (2006) Decay-accelerating factor (CD55): a versatile acting molecule in human malignancies.Biochim Biophys Acta. 1766 (1): 42-52.
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Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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