CD55 Antibody | Bu14

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CD55 Antibody | Bu14 gallery image 1

Staining of human peripheral blood monocytes with Mouse anti Human CD55 (MCA2659)

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CD55 Antibody | Bu14 gallery image 2

Staining of human peripheral blood monocytes with Mouse anti Human CD55:FITC (MCA2659F)

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  • CD55 Antibody | Bu14 thumbnail image 1
  • CD55 Antibody | Bu14 thumbnail image 2
  • Mouse anti Human CD55
  • Mouse anti Human CD55:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    Bu14
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2659FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2659F
    MCA2659Fdatasheet pdfdatasheet pdf0.2 mg
    MCA2659
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD55 antibody, clone Bu14 recognizes human CD55, also known as Decay Accelerating Factor (DAF). CD55 is a glycosylphosphatylinositol (GPI) anchored type I surface protein which prevents complement mediated lysis of autologous cells. CD55 functions to limit the formation and accelerate the decay of C3/C5-convertases from the classic and alternative pathway.

      The molecular weight of CD55 varies between 50 and 100kDa dependent on cell type. Alternative transcripts can lead to soluble DAF, which is detectable in plasma, tears, saliva, urine and cerebrospinal fluid.

      CD55 is the receptor for CD97, Echovirus and Coxsackie B virus, and has been linked to many diseases including multiple sclerosis, rheumatoid arthritis and cancer development.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      soluble fraction
      respiratory burst
      elevation of cytosolic calcium ion concentration
      anchored to membrane
      extracellular region
      innate immune response
      membrane raft
      integral to plasma membrane
      complement activation, classical pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/200
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD55 Antibody Formats

    Formats Clone Applications Sizes available
    CD55 Antibody : FITC Bu14 F 0.1 mg
    CD55 Antibody : Purified Bu14 F 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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