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CD300a antibody | P192

Mouse anti Human CD300a

Product Type
Monoclonal Antibody
Clone
P192
Isotype
IgG2a
Specificity
CD300a

Product Code Applications Pack Size List Price Your Price Qty
MCA5747
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.2 mg loader
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loader

Mouse anti Human CD300a antibody, clone P192 recognizes human CD300a, otherwise known as IRp60 or inhibitory receptor protein 60. CD300a is a ~60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.

Cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils significantly inhibits their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Polyclonal population of NK cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Source Reference

  1. Cantoni, C. et al. (1999) Molecular and functional characterization of IRp60, a member of the immunoglobulin superfamily that functions as an inhibitory receptor in human NK cells.
    Eur J Immunol. 29: 3148-59.

References for CD300a antibody

  1. Bachelet, I. et al. (2005) The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells.
    J Immunol. 175: 7989-95.
  2. Bachelet, I. et al. (2006) Abrogation of allergic reactions by a bispecific antibody fragment linking IgE to CD300a.
    J Allergy Clin Immunol. 117: 1314-20.
  3. Bachelet, I. et al. (2008) Suppression of normal and malignant kit signaling by a bispecific antibody linking kit with CD300a.
    J Immunol. 180: 6064-9.
  4. Munitz, A. et al. (2006) The inhibitory receptor IRp60 (CD300a) suppresses the effects of IL-5, GM-CSF, and eotaxin on human peripheral blood eosinophils.
    Blood. 107:1996-2003.

Synonyms
IRp60
RRID
AB_10960847
UniProt
Q9UGN4
Entrez Gene
CD300A
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity

MCA5747

1605 161120 167009

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