CD294 Antibody

CD294 Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human brain cortex with Rabbit anti Human CD294 (AHP2174)

CD294 Antibody gallery image 2

Staining of formalin fixed, paraffin embedded human adrenal gland with Rabbit anti Human CD294 (AHP2174)

CD294 Antibody gallery image 3

Staining of formalin fixed, paraffin embedded human cerebellum with Rabbit anti Human CD294 (AHP2174)

CD294 Antibody gallery image 4

Western blotting of Jurkat cell lysate probed with Rabbit anti Human CD294 (AHP2174)

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  • Rabbit anti Human CD294 (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2174E, P, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human CD294 antibody recognizes CD294, also known as chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and GRP44. CD294 is a G protein-coupled receptor which has a high affinity for prostaglandin D2 (PGD2).

      CD294 is predominantly expressed on Th2 cells, basophils and eosinophils, and its activation by PDG2 mediates chemotaxis of these cells to inflammatory sites. CD294 may be a useful marker for Th2 cells, and enhances their function and signalling with a possible role in Th2 cell differentiation.

      PGD2 is thought to contribute to bronchoconstriction, eosinophilia and mucus production in allergic asthma, and CD294 may contribute to this disease.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human CD294 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A synthetic peptide corresponding to an 18 amino acid sequence from near the amino terminus of human CD294 – KLH conjugated.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      N-formyl peptide receptor activity
      prostaglandin J receptor activity
      inhibition of adenylate cyclase activity by G-protein signaling pathway
      calcium-mediated signaling
      prostaglandin F receptor activity
      prostaglandin D receptor activity
      positive regulation of G-protein coupled receptor protein signaling pathway
      immune response
      plasma membrane
      integral to membrane
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin5ug/ml
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      *This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

      Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Histology Positive Control Tissue
      Human adrenal gland
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD294 Antibody Formats

    Formats Applications Sizes available
    CD294 Antibody : Purified E, P, WB 50 µg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Histar Detection SystemSTAR3000A50 TestsC, P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human adrenal gland

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