AKT1 Antibody (PrecisionAbTM Antibody) | OTI4C11

//www.bio-rad-antibodies.com/human-akt1-antibody-oti4c11-vma00253.html?RenderPageSpecific=1
AKT1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI4C11 gallery image 1

Western blot analysis of whole cell lysates probed with AKT1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 19 second exposure. Arrow points to AKT1 (molecular weight 60 kDa)

Enlarge
  • AKT1 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI4C11 thumbnail image 1
  • AKT1 Antibody with Control Lysate
  • Mouse anti AKT1
  • AKT1 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    AKT1 antibody recognizes the Rac-alpha serine/threonine-protein kinase, also known as AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha or proto-oncogene c-Akt. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00253WBdatasheet pdfdatasheet pdf100 µl
      VMA00253
      VMA00253KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00253K
      VMA00253KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00253KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human AKT1 antibody recognizes the RAC-alpha serine/threonine-protein kinase, also known as AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt and rac protein kinase alpha.

        The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1 (provided by RefSeq, Jul 2011).

        Mouse anti Human AKT1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti AKT1 detects a band of approximately 60 kDa in Jurkat cell lysates
        Anti AKT1 detects a band of approximately 60 kDa in Jurkat cell lysates
        Anti AKT1 detects a band of approximately 60 kDa in Jurkat cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant human AKT1 (NP_005154) produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        ATP binding
        protein import into nucleus, translocation
        mRNA metabolic process
        positive regulation of nitric oxide biosynthetic process
        positive regulation of glycogen biosynthetic process
        activation-induced cell death of T cells
        identical protein binding
        regulation of neuron projection development
        positive regulation of fat cell differentiation
        platelet activation
        insulin receptor signaling pathway
        protein autophosphorylation
        phosphatidylinositol-3,4-bisphosphate binding
        response to fluid shear stress
        response to UV-A
        nitric oxide biosynthetic process
        peptidyl-serine phosphorylation
        positive regulation of glucose import
        activation of pro-apoptotic gene products
        endocrine pancreas development
        negative regulation of plasma membrane long-chain fatty acid transport
        positive regulation of establishment of protein localization in plasma membrane
        positive regulation of cyclin-dependent protein kinase activity involved in G1/S
        plasma membrane
        phosphatidylinositol-mediated signaling
        G-protein coupled receptor protein signaling pathway
        negative regulation of fatty acid beta-oxidation
        insulin-like growth factor receptor signaling pathway
        nitric-oxide synthase regulator activity
        nerve growth factor receptor signaling pathway
        nucleoplasm
        regulation of translation
        induction of apoptosis by intracellular signals
        glycogen biosynthetic process
        negative regulation of protein kinase activity
        cytosol
        phosphatidylinositol-3,4,5-trisphosphate binding
        response to heat
        positive regulation of nitric-oxide synthase activity
        positive regulation of lipid biosynthetic process
        enzyme binding
        positive regulation of cell growth
        T cell costimulation
        protein serine/threonine kinase activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "AKT1" specificity.