14-3-3 Beta/Alpha Antibody (PrecisionAbTM Antibody)

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14-3-3 Beta/Alpha Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with 14-3-3 beta/alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 82.1 second exposure. Arrow points to 14-3-3 beta/alpha (molecular weight 28 kDa)

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  • 14-3-3 Beta/Alpha Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • 14-3-3 Beta/Alpha Antibody with Control Lysate
  • 14-3-3 Beta/Alpha Antibody with Control Lysate
  • Mouse anti 14-3-3 Beta/Alpha
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    14-3-3 beta/alpha antibody recognizes 14-3-3 protein beta/alpha, also known as 14-3-3 alpha, 14-3-3 protein beta/alpha, brain protein 14-3-3, beta isoform, epididymis secretory protein Li 1, protein 1054, protein kinase C inhibitor protein 1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide or beta polypeptide. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00333WBdatasheet pdfdatasheet pdf100 µl
      VMA00333
      VMA00333KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00333K
      VMA00333KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00333KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human 14-3-3 beta/alpha antibody recognizes the 14-3-3 protein beta/alpha, also known as 14-3-3 alpha, brain protein 14-3-3, beta isoform, epididymis secretory protein Li 1, protein 1054, protein kinase C inhibitor protein 1, protein kinase C inhibitor protein-1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide.

        Encoded by the YWHAB gene, 14-3-3 protein beta/alpha belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for YWHAB (provided by RefSeq, Jul 2008).

        Mouse anti Human 14-3-3 protein beta/alpha antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti 14-3-3 beta/alpha detects a band of approximately 28 kDa in MCF7 cell lysates
        Anti 14-3-3 beta/alpha detects a band of approximately 28 kDa in MCF7 cell lysates
        Anti 14-3-3 beta/alpha detects a band of approximately 28 kDa in MCF7 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Mouse monoclonal antibody prepared by affinity chromatography on Protein G
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant human 14-3-3 beta/alpha
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg MCF7 lysate lyophilized in RIPA buffer.
        400μg MCF7 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        melanosome
        mRNA metabolic process
        Ras protein signal transduction
        centrosome
        axon guidance
        negative regulation of protein dephosphorylation
        cytoplasmic sequestering of protein
        cytosol
        insulin receptor signaling pathway
        protein domain specific binding
        epidermal growth factor receptor signaling pathway
        activation of pro-apoptotic gene products
        histone deacetylase binding
        phosphoserine binding
        nerve growth factor receptor signaling pathway
        activation of MAPKK activity
        induction of apoptosis by intracellular signals
        perinuclear region of cytoplasm
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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