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Bovine interleukin-6 ELISA

Recombinant Bovine interleukin-6 (PBP021) detected using Chicken anti Bovine interleukin-6 (AHP2380) as the capture reagent and Chicken anti Bovine interleukin-6:Biotin (AHP2380B) as the detection reagent followed by Streptavidin:HRP

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  • Recombinant Bovine Interleukin-6
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP021E, FN, WBdatasheet pdfdatasheet pdf5 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant Bovine interleukin-6 (IL-6) is a recombinant protein corresponding to aa 30-208 of native bovine IL-6.

      Bovine interleukin-6 is a multifunctional cytokine secreted by a range of cell types, in particular T lymphocytes and macrophages. IL-6 has a range of activities and includes the differentiation of B cells into Ig producing cells, differentiation of lymphocytes and of monocytes. IL-6 functions in both a pro-inflammatory role where it is an important inducer of the accute phase response and also as an anti-inflamatory myokine.

      Interleukin-6 induces myeloma growth and is an essential cytokine required for successful hybridoma growth.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilized
    • Reconstitution
      Reconstitute with 20 µl sterile PBS containing 0.1% carrier protein
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
    • Preservative Stabilisers
      10% Trehalose
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
      20.7 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at -20oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      cytokine activity
      immune response
      extracellular space
      growth factor activity
      acute-phase response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2380 as a coating antibody and AHP2380B as a detection antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-6 Formats

    Formats Applications Sizes available
    IL-6 : Rec. Protein E, FN, WB 5 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IL-6 thumbnail image 1

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