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IL-6 Antibody

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Bovine interleukin-6 ELISA

Recombinant Bovine interleukin-6 (PBP021) detected using Chicken anti Bovine interleukin-6 (AHP2380) as the capture reagent and Chicken anti Bovine interleukin-6:Biotin (AHP2380B) as the detection reagent followed by Streptavidin:HRP

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  • Chicken anti Bovine Interleukin-6
  • Chicken anti Bovine Interleukin-6:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2380E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2380BEdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Chicken anti Bovine interleukin-6 antibody recognizes bovine interleukin-6 (IL-6), a 23.8 kDa cytokine belonging to the IL-6 superfamily.

      Bovine interleukin-6 is a multifunctional cytokine secreted by a range of cell types, in particular T lymphocytes and macrophages. IL-6 has a range of activities and includes the differentiation of B cells into Ig producing cells, differentiation of lymphocytes and of monocytes. IL-6 functions in both a pro-inflammatory role where it is an important inducer of the accute phase response and also as an anti-inflamatory myokine.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to bovine interleukin-6 were raised by repeated immunisation of chickens with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant bovine interleukin-6
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch.
    • GO Terms
      growth factor activity
      extracellular space
      cytokine activity
      immune response
      acute-phase response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.1ug/ml2ug/ml
    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a capture antibody in a sandwich ELISA together with AHP2380B as the detection reagent. Recombinant Bovine interleukin-6 (PBP021) may be used as a standard.
    • ELISA
      This product may be used as a detection antibody in a sandwich ELISA together with AHP2380 as the capture reagent. Recombinant Bovine interleukin-6 (PBP021) may be used as a standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-6 Antibody Formats

    Formats Applications Sizes available
    IL-6 Antibody : Purified E, WB 0.1 mg
    IL-6 Antibody : Biotin E 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

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