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IgG2

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PBP004
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Purified bovine IgG2 is prepared from normal bovine serum by fractionantion followed by DEAE chromatography.
By immunoelectrophoresis, this preparation has been shown to react with antiserum specific for bovine IgG2. No reactivity was seen with antiserum specific for bovine IgG1, IgA or IgM.

This product contains <1% IgG1, IgA and IgM as determined by ELISA.

Target Species
Bovine
Product Form
Purified IgG2 - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgG2

  1. Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for
    Anaplasma marginale
    major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
    J Immunol. 174 (11): 6702-15.

RRID
AB_324782

PBP004

155032 160124

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