Mouse IgG2a Negative Control antibody
Mouse IgG2a Negative Control:APC
- Product Type
- Negative/Isotype Control
- Isotype
- IgG2a
- Specificity
- Mouse IgG2a Negative Control
Mouse IgG2a negative control antibody, clone OX-34 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that MCA929 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues. This antibody may not be suitable for intracellular staining on some cell types. |
- Target Species
- Negative Control
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin 5% Sucrose - Immunogen
- Activated rat T-helper cells.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) APC 650 661 - Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | * |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Mouse IgG2a Negative Control antibody
-
Avigdor, A. et al. (2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow.
Blood. 103 (8): 2981-9. -
Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
Avian Pathol. 45 (2): 235-43. -
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20. -
Terpeluk, R.E. et al. (2024) Supplementation of Foals with a Saccharomyces cerevisiae Fermentation Product Alters the Early Response to Vaccination
Animals. 14 (6): 960. -
Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
Vet Immunol Immunopathol. 254: 110520. -
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366.
- RRID
- AB_322306
MCA929APC
150483If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Negative Control ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up