HuCAL Fab-dHLX-MH Negative Control antibody | AbD04652

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HuCAL Fab-dHLX-MH Negative Control Antibody, clone AbD04652 gallery image 1 — Figure A. RPE conjugated mouse anti bovine CD32 (MCA5918PE) and FITC conjugated HuCAL isotype control (**HCA052F**). Figure B. RPE conjugated mouse anti bovine CD32 (MCA5918PE) and FITC conjugated mouse anti bovine CD282 (HCA152F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells

HuCAL Fab-dHLX-MH Negative Control:Alexa Fluor® 647

HuCAL Fab-dHLX-MH Negative Control:FITC

HuCAL Fab-dHLX-MH Negative Control

Product Type: Negative/Isotype Control
Clone: AbD04652
Isotype: HuCAL Fab bivalent

Product Code	Applications	Pack Size
HCA052A647	E F	1 ml
HCA052F	C E F P WB	0.1 mg
HCA052A	C E P WB	50 µg
HCA052	C E F P WB	0.1 mg

Summary
Secondary Antibodies

HuCAL Fab-dHLX-MH negative control, clone AbD04652 is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a negative control reagent when using other HuCAL antibodies of the same format.

It is recommended that this reagent is used at the same concentration as the test reagent.

Product Details

Target Species: Negative Control
Product Form: A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain. This antibody is supplied as a liquid conjugated to Alexa Fluor® 647.
Product Form: A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain, and conjugated to fluorescein isothiocyanate.
Product Form: Pack Size: 50 µg
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.

Pack Size: 0.1 mg
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation: Metal chelate affinity chromatography
Preparation: Metal chelate affinity chromatography
Preparation: Metal chelate affinity chromatography
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09%Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: Pack Size: 50 µg
0.09%Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Pack Size: 0.1 mg
0.01% Thiomersal
Immunogen: Green fluorescent protein
Approx. Protein Concentrations: Antibody concentration 0.05mg/ml
Approx. Protein Concentrations: Antibody concentration 0.1mg/ml
Approx. Protein Concentrations: Pack Size: 50 µg
Antibody concentration 0.1mg/ml following reconstitution

Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage: Pack Size: 50 µg
Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.

Pack Size: 0.1 mg
Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 6 months from date of despatch.

More Information

Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents.

His-tag is a registered trademark of EMD Biosciences.

This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use: For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory: For research purposes only

Applications of HuCAL Fab-dHLX-MH Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Human IgG F(ab')2:HRP	0500-0099	0.5 ml	E WB
Mouse anti Histidine Tag:HRP	MCA1396P	0.1 mg	E WB
Mouse anti c-Myc:HRP	MCA2200P	0.1 mg	C E P WB*
Goat anti Human IgG F(ab')2:HRP	0500-0099	0.5 ml	E WB
Mouse anti c-Myc:HRP	MCA2200P	0.1 mg	C E P WB*
Mouse anti Penta Histidine Tag:HRP	MCA5995P	125 µl	E WB

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