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Goat anti Escherichia coli antibody (OBT986) used to evaluate E. coli colonization in murine intestinal mucosa by immunofluorescence.
Western diet favors emergence of E. coli associated with ileal, cecal and colonic mucosa.
(A) E. coli bacteria were isolated from the mucosa by plating on Drigalski agar plates and the number of bacteria was determined by counting the CFU. (B) E. coli colonization of the ileal mucosa of CEABAC10?mice under a conventional or an HF/HS diet was visualized by confocal microscopy and quantification was performed by assessing global fluorescence intensities using the ImageJ software. ****P?<0.0001.
From: Agus A, Denizot J, Thévenot J, Martinez-Medina M, Massier S, Sauvanet P, Bernalier-Donadille A, Denis S, Hofman P, Bonnet R, Billard E, Barnich N.
Western diet induces a shift in microbiota composition enhancing susceptibility to Adherent-Invasive E. coli infection and intestinal inflammation.
Sci Rep. 2016 Jan 8;6:19032.