Anti-Guselkumab Antibodies
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Bioanalytical Antibodies
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Antibodies for Bioanalysis and Drug Monitoring
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Our Anti-Biotherapeutic Antibodies
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Antibodies for Bioanalysis and Drug Monitoring
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Antibodies for bioanalysis and drug monitoring of guselkumab and biosimilars
Develop highly selective and sensitive PK and ADA assays for guselkumab (Tremfya®) using our range of ready-made anti-idiotypic antibodies.
- Inhibitory and non-inhibitory antibodies specific to guselkumab
- Fully human surrogate positive control or calibrator
- Sequence defined, well characterized reagents with secure supply
- Recombinant production, stringent quality control for batch-to-batch consistency
- Non-animal-derived antibodies, reducing the use of animals in science
Table 1. Antibodies Specific to Guselkumab and Guselkumab-IL-23A complex.
Specificity |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
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|---|---|---|---|---|---|---|---|
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Guselkumab Inhibitory Type 1 |
HCA420 |
AbD59938ia |
Human IgG1 |
0.06 |
ADA assay |
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HCA421 |
AbD59947ia |
Human IgG1 |
0.3 |
ADA assay |
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TZA070 |
AbD59307ad |
Fab-F-Spy2-H1 |
0.06
|
PK bridging ELISA (capture) |
|||
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TZA071 |
AbD59937ad AbD59937pap |
Fab-F-Spy2-H1 Fab2-FH-X22-HRP2 |
0.06
0.06 |
PK bridging ELISA (detection) |
|||
|
Guselkumab-Human IL-23A Complex Type 3 |
TZA072 TZA072P |
AbD59967ad AbD59967pap |
Fab-F-Spy2-H1 Fab2-FH-X22-HRP2 |
33 33 |
PK ELISA antigen capture format |
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* Affinity measured in the monovalent Fab format.
1 Fab-F-Spy2-H = Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
2 Fab2-FH-X22-HRP = Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags, and Fab-SpyTag coupled to HRP-conjugated BiCatcher.
Anti-Guselkumab Inhibitory Antibodies (Type 1)
Type 1 anti-guselkumab antibodies inhibit the binding of the drug guselkumab to its target, human interleukin-23 (IL-23). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.
Anti-Guselkumab-IL-23A Complex Specific Antibodies (Type 3)
The Type 3 antibodies specifically recognize the drug-target complex, detecting guselkumab only when it is bound to IL-23. They are suitable as a detection antibody in a PK antigen capture assay.
ELISA Protocols to Get You Started
PK Bridging ELISA
Fig. 1. Guselkumab PK bridging ELISA using antibodies TZA070 (capture) and TZA71P (detection).
Schematic image of PK bridging ELISA detecting free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Fab format (purple), labeled with HRP.
PK Antigen Capture
Fig. 2. Guselkumab PK antigen capture ELISA using IL-23A and TZA072P.
Schematic image of PK antigen capture ELISA. Drug target (red), monoclonal antibody drug (gold), drug-target complex detection antibody, monovalent Fab format (purple), labeled with HRP.
ADA Bridging ELISA
Fig. 3. Guselkumab ADA bridging ELISA using antibodies HCA420 and HCA421.
Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).
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