Histone H4 Acetylated Antibody

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Histone H4 Acetylated Antibody gallery image 1

Published customer image:
Rabbit anti acetylated Histone H4 antibody (AHP418) used for the evaluation of acetylated histone H4 expression in rat intestine by western blotting.
Image caption:
Acetylation on histone 4 in the trinitrobenzene sulfonic acid (TNBS) rat model of inflammation. A: Sham (saline treated) operated and TNBS treated rat large intestine. Rats were Sham or TNBS treated for 7 days before sacrifice. Well-advanced inflammation is apparent in the colon of the TNBS rat model. B: Pan acetylation on histone 4 (H4). The Sham model was saline-treated and therefore less inflamed (control). Results were obtained by Western blotting. The ratio of the density of histone H4 bands over β-actin control bands was calculated. In order to evaluate changes in density from different Western blotting experiments control densitometry was denoted as 100% and differences were accounted as increase percentage of the control. Representative examples of bands obtained are also illustrated. Columns represent the densitometric evaluation of three independent experiments (mean ± SEM). (*p <0.05 vs Sham proximal or Sham distal respectively).

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patternsof histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.

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Histone H4 Acetylated Antibody gallery image 2

Published customer image:
Rabbit anti acetylated Histone H4 antibody (AHP418) used for the evaluation of acetylated histone H4 expression in rat intestine by western blotting.
Image caption:
Acetylation on histones 3 (H3) and 4 (H4) in Lewis and Sprague-Dawley dextran sulfate sodium (5% DSS) treated rats. Tissue samples were obtained from the sigmoid colon of the animals. A: Representative bands of H4 and H3 acetylation as obtained by Western blotting. β-actin levels were measured to ensure equal protein loading. The results are representative of three independent experiments. B, C: Graphical analysis of data Lanes represent: (1) non-DSS treated Lewis rats (control), (2) DSS-treated Lewis rats, (3) non-DSS treated Sprague-Dawley rats (control) (4) DSS-treated Sprague-Dawley rats. Columns represent the mean ± SEM of three independent experiments (*p <0.05). D: Histone 3 (H3) and histone 4 (H4) localisation in Peyer's patches of dextran sulfate sodium (DSS) treated Lewis rats. H3 is acetylated mainly in the mantle zone and H4 is acetylated throughout the surface of Peyer's patches to both mantle zone and germinal centre cells. In Peyer's patches of untreated animals no acetylation on either histone 3 or 4 was apparent. Micrographs are representative of two individual experiments for each strain. Isotype controls show no staining.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patternsof histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.

Enlarge
Histone H4 Acetylated Antibody gallery image 3

Published customer image:
Rabbit anti acetylated Histone H4 antibody (AHP418) used for the evaluation of acetylated histone H4 expression in human Chron's disease intestinal tissue by western blotting.
Image caption:
Acetylation on histone 4 (H4) and H4 lysine residues in Crohn's disease. Columns represent the mean ± SEM of three independent experiments. Four biopsies were pooled to obtain sufficient protein for one experiment (50 μg of protein) (*p <0.05 vs control). Pan acetylation on H4 in Crohn's disease (A). Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (B), K8 (C), K12 (D), and 16 (E), in non-inflamed, inflamed tissue and Peyer's patches of Crohn's disease patients. Results were obtained by Western blotting. Columns represent the mean ± SEM of three independent experiments. (*p <0.05 vs control, #p <0.005 vs non-inflamed CD). Representative images of the bands obtained are illustrated.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patternsof histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.

Enlarge
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP418CH, E, IF, IP, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP418
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Histone H4 (Acetylated) antibody was raised against the tetra-acetylated form of histone H4 and recognizes all acetylated isoforms of histone H4.
    • Intended Use
    • Target Species
      Synthetic Peptide
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MammalsExpected from Sequence
      Drosophilayes
      Yeastyes
      PlantsExpected from Sequence
      AmphibiaExpected from Sequence
      Humanyes
      Arabidopsisyes
      Mouseyes
      Zebrafishyes
      Wheatyes
      Asellus aquaticusyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      KLH-conjugated peptide acetylated at lysines 5,8,12,16: NSGRGAcKGGAcKGLGAcKGGAAcKRHYCC.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      negative regulation of megakaryocyte differentiation
      CenH3-containing nucleosome assembly at centromere
      nucleosome
      DNA binding
      protein binding
      phosphatidylinositol-mediated signaling
      telomere maintenance
      nucleoplasm
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Chromatin Immunoprecipitation
      ELISA1/400
      Immunofluorescence1/200
      Immunoprecipitation
      Western Blotting1/800

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Histone H4 Acetylated Antibody Formats

    Formats Applications Sizes available
    Histone H4 Acetylated Antibody : Serum CH, E, IF, IP, WB 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Western Blotting

              References

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