Histone H4 Ac5 Antibody

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Histone H4 Ac5 Antibody gallery image 1

Published customer image:
Rabbit anti Histone H4 (Ac5) antibody used for the detection of acetylated histone H4 in rat colitis tissue lysates by western blotting.
Image caption:
Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (A), 8 (K8) (B), 12 (K12) (C) and 16 (K16) (D) in a Sham (control) and trinitrobenzene sulfonic acid (TNBS) rat model of colitis. Results were obtained by Western blotting. In order to evaluate changes in density from different western blotting experiments control densitometry was denoted as 100% and differences were accounted as increase percentage of the control. Representative examples of bands obtained are also illustrated. Columns represent the densitometric evaluation of three independent experiments (mean ± SEM). (*p < 0.05 vs Sham proximal or Sham distal respectively).

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.

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Published customer image:
Rabbit anti Histone H4 (Ac5) antibody used for the detection of acetylated histone H4 in Dextran sulphate treated rat tissue lysates by western blotting.
Image caption:
Acetylation on histone 4 (H4) specific lysine residues 5 (K5), 8 (K8), 12 (K12) and 16 (K16) in Lewis and Sprague-Dawley dextran sulfate sodium (5% DSS). A: Representative bands of H4K5, K8, K12 and K16 acetylation. Lanes for Lewis rats represent: non-DSS treated (control) and DSS-treated. Likewise representative bands are illustrated for the Sprague-Dawley rats. Graphical representation of Western blotting data. H4 acetylation of K5 (B), K8 (C), K12 (D) and K16 (E). Columns represent the mean ± SEM (bar) of three independent experiments.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.

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Histone H4 Ac5 Antibody gallery image 3

Published customer image:
Rabbit anti Histone H4 (Ac5) antibody used for the detection of acetylated histone H4 in intestinal tissue lysates by western blotting.
Image caption:
Acetylation on histone 4 (H4) and H4 lysine residues in Crohn's disease. Columns represent the mean ± SEM of three independent experiments. Four biopsies were pooled to obtain sufficient protein for one experiment (50 µg of protein) (*p < 0.05 vs control). Pan acetylation on H4 in Crohn's disease (A). Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (B), K8 (C), K12 (D), and 16 (E), in non-inflamed, inflamed tissue and Peyer's patches of Crohn's disease patients. Results were obtained by Western blotting. Columns represent the mean ± SEM of three independent experiments. (*p < 0.05 vs control, #p < 0.005 vs non-inflamed CD). Representative images of the bands obtained are illustrated.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.

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Histone H4 Ac5 Antibody gallery image 4

Published customer image:
Rabbit anti Histone H4 (Ac5) antibody used for the detection of acetylated histone H5 in 10T1/2 cells by immunofluorescence.
Image caption:
Spatial Relationship between CBP and Specific Histone H3 Post-Translational Modifications The spatial relationship between CBP and a known product of its HAT activity, acetylated K5 (H4) (A), or an unrelated histone H3 modification, trimethylated K4 (H3) (B), was investigated. Representative and deconvolved high-resolution images (100×) of interphase 10T1/2 cells immunofluorescently labeled with anti-CBP and either anti-AcK9 or anti-tMeK4 counterstained with DAPI are presented. The merged image presents a single plane from the 3-D projection where CBP and AcK9 or tMeK4 are shown in green and red, respectively. A region, identified by the white box, is further magnified to show the spatial relationship between CBP and either post-translational histone modification. Scale bars represent 3 μm.

From: McManus KJ, Stephens DA, Adams NM, Islam SA, Freemont PS, Hendzel MJ (2006) The Transcriptional Regulator CBP Has Defined Spatial Associations within Interphase Nuclei.
PLoS Comput Biol 2(10): e139.

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  • Rabbit anti Histone H4 (Ac5)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP962CH, E, IF, IP, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP962
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Histone H2 (Ac5) antibody recognizes Histone H4 when acetylated at lysine 5.
    • Intended Use
    • Target Species
      Synthetic Peptide
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mammalsyes
      Drosophilayes
      Amphibiayes
      Yeastyes
      Plantsyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Ovalbumin-conjugated peptide: NSGRGAcKGGKGLCc.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      telomere maintenance
      DNA binding
      nucleosome
      CenH3-containing nucleosome assembly at centromere
      nucleoplasm
      protein binding
      phosphatidylinositol-mediated signaling
      negative regulation of megakaryocyte differentiation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Chromatin Immunoprecipitation
      ELISA1/1000
      Immunofluorescence1/400
      Immunoprecipitation
      Western Blotting1/600

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Histone H4 Ac5 Antibody Formats

    Formats Applications Sizes available
    Histone H4 Ac5 Antibody : Serum CH, E, IF, IP, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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