Tyrosine Hydroxylase (pSer40) Antibody

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Tyrosine Hydroxylase (pSer40) Antibody gallery image 1

Western blot of phospho- and dephospho-tyrosine hydroxylase demonstrating the selective immunolabeling by the phospho-specific TH Ser40 antibody

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  • Rabbit anti Tyrosine Hydroxylase (pSer40)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP912C, IF, WB*datasheet pdfdatasheet pdf0.1 ml
    AHP912
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti phosphorylated tyrosine hydroxylase antibody recognizes tyrosine hydroxylase, when phosphorylated at serine 40.

      Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.

      Phosphorylation of TH has been shown to occur at several serine residues. Phosphorylation at serine 40 results in an increase in hydroxylase activity, and phosphorylation at serine 19 is reported to promote phosphorylation of the serine 40 residue (Dunkley et al. 2004).

      Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson’s disease (Pearson et al 1979).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/1000
      Immunohistology - Frozen1/1000
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP912 detects a band of approximately 60kDa in PC-12 cell lysates, following stimulation by Okadaic acid.
    • Instructions For Use

    Additional Tyrosine Hydroxylase (pSer40) Antibody Formats

    Formats Applications Sizes available
    Tyrosine Hydroxylase (pSer40) Antibody : Purified C, IF, WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Western Blotting

              References

              Further Reading

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