TGN38 Antibody

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TGN38 Antibody gallery image 1

Published customer image:
Sheep anti Rat TGN38 antibody used for identification of the trans-golgi network in mouse embryonic fibroblasts by immunofluorescence.
Image caption:
Phosphorylated TBK1 is localized to the TGN in ISD-stimulated primary MEFs. a, Immunostaining of phosphorylated TBK1 (red) in cells treated with nocodazole and ISD. GM130 (a CGN protein, cyan) and TGN46 (a TGN protein, magenta) were co-stained. Scale bars, 10 μm. b-c, The mini-Golgi stacks indicated by the boxes in (a) were magnified. The cis and trans-regions of the mini-Golgi stacks were outlined with dashed line.

From: Mukai, K. et al. 2016.
Activation of STING required Palmtoylation oat the Golgi.
Nat Comms article 11932.

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  • TGN38 Antibody thumbnail image 1
  • Sheep anti Rat TGN38
  • Sheep anti Rat TGN38
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP499C, EM, IF, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP499
    AHP499GC, WBdatasheet pdfdatasheet pdf25 µg
    AHP499G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Rat TGN38 antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the Trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphrey et al. 1993).

      TGN38 is likely to have a role in intracellular transport (McNamara et al. 2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalam et al. 1996).
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      serum diluted - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat TGN38 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Recombinant fusion protein corresponding to extracellular domain of TGN38.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immuno-electron Microscopy
      Immunofluorescence
      Immunohistology - Frozen1/1001/200
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen0.1ug/ml1ug/ml
      Western Blotting0.1ug/ml1ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
      Fixation with methanol or methanol/acetone recommended.
    • Immunohistology
      Fixation with methanol or methanol/acetone recommended.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional TGN38 Antibody Formats

    Formats Applications Sizes available
    TGN38 Antibody : Purified C, WB 25 µg
    TGN38 Antibody : Serum C, EM, IF, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immuno-electron Microscopy
                Immunofluorescence

              References

              Further Reading

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