MUNC-18 (pSer515) Antibody

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MUNC-18 (pSer515) Antibody gallery image 1

Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~65 kDa Munc-18 protein phosphorylated at Ser515

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  • MUNC-18 (pSer515) Antibody thumbnail image 1
  • Rabbit anti MUNC-18 (pSer515)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1234WB*datasheet pdfdatasheet pdf0.1 ml
    AHP1234
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Rat MUNC-18 (pSer515) antibody recognizes MUNC-18, also known as syntaxin-binding protein 1, N-Sec1, protein unc-18 homolog 1 (Unc18-1) and p67, when phosphorylated at serine 515. MUNC-18 is a ~67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter release, facilitating synaptic vesicle docking and fusion. It inhibits the formation of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex by binding syntaxin 1 preventing the other components (synaptobrevin and synaptosomal associated protein of 25 kDa (SNAP-25)) from binding. The function of Munc-18 is regulated by the phosphorylation of serine 515 by protein kinase C.

      Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      DogExpected from Sequence
      XenopusExpected from Sequence
      HumanExpected from Sequence
      BovineExpected from Sequence
      ChickenExpected from Sequence
      MouseExpected from Sequence
      PrimateExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat Munc-18 (pSer515) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      50% Glycerol
      0.01%Bovine Serum Albumin
    • Immunogen
      Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated Ser515 of Munc-18.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM Hepes pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      plasma membrane
      vesicle docking involved in exocytosis
      protein N-terminus binding
      syntaxin-1 binding
      regulation of synaptic vesicle priming
      cytosol
      protein complex
      protein stabilization
      protein transport
      protein domain specific binding
      identical protein binding
      protein kinase binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1234 detects a band/s of approximately 65kDa in rat cortex cell lysates
    • Instructions For Use

    Additional MUNC-18 (pSer515) Antibody Formats

    Formats Applications Sizes available
    MUNC-18 (pSer515) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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