Rat IgG2c Negative Control Antibody

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Rat IgG2c Negative Control Antibody gallery image 1

Figure A. PE conjugated Mouse anti Canine CD25 (MCA5916F) and A488 conjugated Rat IgG2c isotype control (MCA2879A488). Figure B. PE conjugated Mouse anti Canine CD25 (MCA5916F) and A488 conjugated Rat anti Canine CD11/18 (MCA1040PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • Rat IgG2c Negative Control Antibody thumbnail image 1
  • Rat IgG2c Negative Control:Alexa Fluor® 488
  • Rat IgG2c Negative Control
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  • Product Type
    Negative/Isotype Control
  • Isotype
    IgG2c
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2879Fdatasheet pdfdatasheet pdf0.25 mg
    MCA2879
    MCA2879A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2879A488
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat IgG2c negative control antibody is suitable for use as a negative control on human and dog tissues where rat monoclonals of isotype IgG2c are used.
    • Intended Use
    • Target Species
      Negative Control
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Isolated C3H thymic stromal cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.56 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry*
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry*

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. *This antibody should be used at the same concentration as the test antibody.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      *It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Rat IgG2c Negative Control Antibody Formats

    Formats Applications Sizes available
    Rat IgG2c Negative Control Antibody : Purified F 0.25 mg
    Rat IgG2c Negative Control Antibody : Alexa Fluor® 488 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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