IgG2b Antibody

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IgG2b Antibody gallery image 1

Published customer image:
Horseradish peroxidase conjugated Goat anti Rat IgG2b antibody used as a secondary antibody for the detection of pentraxin 3 in mouse tissues by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
PTX3 expression is up-regulated following RRV infection in murine model. (A) 21-day-old C57BL/6 WT mice (n = 4–5 per group) were subcutaneously injected with 104 PFU of RRV or PBS (mock). Mice were sacrificed at 2, 5, 10 and 15 dpi. Serum, quadriceps and ankle joints were harvested. PTX3 expression in serum of RRV- or mock-infected mice was determined by ELISA. (B) Transcriptional profile of PTX3 in quadriceps and ankle joint harvested from RRV- or mock-infected mice at various time points were determined by qRT-PCR. Data were normalized to HPRT and shown as fold expression relative to mock-infected. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA, Bonferroni post-test. Data are presented as mean ± SEM and are representative of 2 independent experiments. (C) Histology of RRV-induced inflammation in quadriceps of WT mice was analyzed by IHC staining with anti-PTX3 antibody at 10 dpi. Arrows indicate abundance of inflammatory infiltrates. Images were taken at 20× magnification. Scale bar, 40 µm. (D) 21-day-old C57BL/6 WT (n = 2–3 per group) mice were infected subcutaneously with 104 PFU RRV. Spleens were harvested at 2 dpi and were characterized and quantified by flow cytometry using the markers as described in Materials and Methods to determine mean fluorescence intensity (MFI) of PTX3 expression in total leukocytes, inflammatory monocytes, neutrophils, NK cells, T cells and B cells. Data are presented as mean ± SEM. **P < 0.01, ***P < 0.001, two-way ANOVA, Bonferroni post-test.

From: Foo S-S, Chen W, Taylor A, Sheng K-C, Yu X, et al. (2015) Role of Pentraxin 3 in Shaping Arthritogenic Alphaviral Disease: From Enhanced Viral Replication to Immunomodulation. PLoS Pathog 11(2): e1004649.

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  • IgG2b Antibody thumbnail image 1
  • Goat anti Rat IgG2b:Alk. Phos.
  • Goat anti Rat IgG2b:HRP
  • Goat anti Rat IgG2b:FITC
  • Goat anti Rat IgG2b
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR114ABE, WBdatasheet pdfdatasheet pdf0.5 mg
    STAR114AB
    STAR114PC, E, P, WBdatasheet pdfdatasheet pdf0.5 mg
    STAR114P
    STAR114FFdatasheet pdfdatasheet pdf0.5 mg
    STAR114F
    STAR114E, F, WBdatasheet pdfdatasheet pdf0.5 mg
    STAR114
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Rat IgG2b antibody recognizes rat IgG2b as assessed by immunoelectrophoresis and ELISA.

      Goat anti Rat IgG2b antibody may cross react with IgG2b from other species.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat IgG2b were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
      Western Blotting1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
      Immunohistology - Frozen1/2001/500
      Immunohistology - Paraffin1/2001/500
      Western Blotting1/10001/30000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/5001/1000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1ug/ml10ug/ml
      Flow Cytometry
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to stain 1 x 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG2b Antibody Formats

    Formats Applications Sizes available
    IgG2b Antibody : Purified E, F, WB 0.5 mg
    IgG2b Antibody : FITC F 0.5 mg
    IgG2b Antibody : Alk. Phos. E, WB 0.5 mg
    IgG2b Antibody : HRP C, E, P, WB 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

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          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
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          BUF057A
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          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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