IgG Antibody

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IgG Antibody gallery image 1

Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b (MCA2293) at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha (MCA78G) is included as a loading control. Detection is with Goat anti Rat IgG:Dylight® (STAR71D800GA)

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IgG Antibody gallery image 2

Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Mouse anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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IgG Antibody gallery image 3

Figure A. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649) and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a PE (STAR71D649) and Mouse anti Canine CD3 FITC (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • IgG Antibody thumbnail image 3
  • Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)
  • Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
  • Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)
  • Goat anti Rat IgG (Mouse Adsorbed)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR71D649GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR71D649GA
    STAR71D800GAF, IF, WBdatasheet pdfdatasheet pdf0.1 mg
    STAR71D800GA
    STAR71D549GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR71D549GA
    STAR71E, Fdatasheet pdfdatasheet pdf0.5 mg
    STAR71
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Rat IgG (Mouse Adsorbed) antibody recognizes rat IgG. Cross-reactivity with mouse IgG has been minimised by adsorption.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to DyLight®649 - liquid
      Purified IgG conjugated to DyLight®800 - liquid
      Purified IgG conjugated to DyLight®549 - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Rat IgG
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/500
      Immunofluorescence1/1001/500
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
      Western Blotting1/100001/50000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/400
      Immunofluorescence1/501/400
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry5ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Purified E, F 0.5 mg
    IgG Antibody : DyLight®649 F, IF 0.1 mg
    IgG Antibody : DyLight®800 F, IF, WB 0.1 mg
    IgG Antibody : DyLight®549 F, IF 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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