FITC conjugated Goat (F(ab′)2 anti Rat IgG antibody recognises rat IgG. Cross-reactivity with mouse IgG has been reduced by adsorption, however, we recommend dilution in buffer containing 10% (v/v) normal mouse serum for use upon mouse tissues.
F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antisera to rat IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Bovine Serum Albumin
Approx. Protein Concentrations
F(ab')2 concentration 0.7 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
1. Fowles, L. et al. (2000) Regulation of urokinase plasminogen activator gene transcription in the RAW264 murine macrophage cell line by macrophage colony-stimulating factor (CSF-1) is dependent upon the level of cell surface receptor. Biochem. J. 347: 313 - 320.
2. Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10. Br J Cancer. 106: 883-888