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IgG Antibody

IgG Antibody gallery image 1

Published customer image:
F(ab')2 Rabbit anti Rat IgG antibody used for the detection of bound rat antibodies to Mouse CD14 and beta actin in western blotting
Image caption:
Transfection system establishment.
A Western blot analysis of CD14 expression in untreated and LPS stimulated murine RAW264.7 macrophages and NIH/3T3 fibroblasts, β-actin as endogeous control. Transfection efficiency test by determination of GFP-positive RAW264.7 cells by cyto fluorescence 24 hours after transfection with B Xtreme Gene HP, C Xtreme Gene 9 DNA, D Superfect, E Polyfect, and F by β-galactosidase assay, ***: p < 0,05.

From: Zschemisch N-H, Brüsch I, Hambusch A-S, Bleich A (2016)
Transcription Factor SP2 Enhanced the Expression of Cd14 in Colitis-Susceptible C3H/HeJBir.
PLoS ONE 11(5): e0155821.

  • IgG Antibody thumbnail image 1
  • Rabbit F(ab')2 anti Rat IgG:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR21BC, E, P, REdatasheet pdfdatasheet pdf1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Horseradish peroxidase conjugated Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat immunoglobulin G. Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method.

      Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography of serum
    • Preservative Stabilisers
      HRP stabiliser (BUF052A)
      0.01% Thiomersal
    • Immunogen
      Purified rat immunoglobulin G.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/50
      Immunohistology - Paraffin1/50
      Immunohistology - Resin1/50

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : HRP C, E, P, RE 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          TMB Signal+BUF054A100 mlE
          TMB CoreBUF056A100 mlE
          TMB Core PrestainedBUF057A100 mlE
          TMB Core+BUF062A100 mlE
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IgG Antibody thumbnail image 1
              • Application NameReference Images
                Immunohistology - Paraffin


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