CD3 Antibody | 1F4

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CD3 Antibody | 1F4 gallery image 1

Staining of paraffin embedded rat spleen with Mouse anti Rat CD3 (MCA772)

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  • CD3 Antibody | 1F4 thumbnail image 1
  • Mouse anti Rat CD3:Alexa Fluor® 647
  • Mouse anti Rat CD3:Alexa Fluor® 700
  • Mouse anti Rat CD3:Biotin
  • Mouse anti Rat CD3:FITC
  • Mouse anti Rat CD3
  • Mouse anti Rat CD3
  • Mouse anti Rat CD3:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
    CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA.
    • Product Type
      Monoclonal Antibody
    • Clone
      1F4
    • Isotype
      IgM
    5 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA772BFdatasheet pdfdatasheet pdf0.1 mg
      MCA772B
      MCA772GAC, F, IP, P *datasheet pdfdatasheet pdf0.1 mg
      MCA772GA
      MCA772FFdatasheet pdfdatasheet pdf0.1 mg
      MCA772F
      MCA772C, F, IP, P *datasheet pdfdatasheet pdf0.2 mg
      MCA772
      MCA772A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA772A647
      MCA772A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA772A700
      MCA772FAFdatasheet pdfdatasheet pdf50 µg
      MCA772FA
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a ~25 kDa antigen which is found on rat T-cells. Mouse anti Rat CD3, clone 1F4 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex.

        Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.
      • Intended Use
      • Target Species
        Rat
      • Product Form
        Purified IgM conjugated to Alexa Fluor® 647 - liquid
        Purified IgM conjugated to Alexa Fluor® 700 - liquid
        Purified IgM conjugated to Biotin - liquid
        Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgM - liquid
        Purified IgM - liquid
        Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      • Reconstitution
      • Preparation
        Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
        Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Immunogen
        F344 rat T cells stimulated with PMA (TPA) and calcium ionophore
      • Purity
      • Approx. Protein Concentrations
        IgM concentration 0.05 mg/ml
        IgM concentration 0.05 mg/ml
        IgM concentration 0.1 mg/ml
        IgM concentration 0.1 mg/ml
        Pack Size: 0.2 mgIgM concentration 1.0 mg/ml
        Pack Size: 0.1 mgIgM concentration 1 mg/ml
        Pack Size: 0.2 mgIgM concentration 1.0 mg/ml
        Pack Size: 0.1 mgIgM concentration 1 mg/ml
        IgM concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised BALB/c mice were fused with cells of the P3-X63-Ag8.653 mouse myeloma cell line.
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.2 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.2 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
        cell surface receptor linked signaling pathway
        positive thymic T cell selection
        establishment or maintenance of cell polarity
        protein transport
        transmembrane receptor activity
        integral to membrane
        protein heterodimerization activity
        regulation of apoptosis
      • UniProt
      • Entrez Gene
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
        NeatPack Size: 0.1 mg
        1/5Pack Size: 50 µg
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/101/25
        Immunohistology - Frozen1/101/25
        Immunoprecipitation
        Immunohistology - Paraffin(1)1/10
        (1)
        This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B, refer to McKechnie N.M. et al. for details.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/101/25
        Immunohistology - Frozen1/101/25
        Immunoprecipitation
        Immunohistology - Paraffin(1)1/10
        (1)
        This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B, refer to McKechnie N.M. et al. for details.
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
        NeatPack Size: 0.1 mg
        1/5Pack Size: 50 µg

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional CD3 Antibody Formats

      Formats Clone Applications Sizes available
      CD3 Antibody : Alexa Fluor® 647 1F4 F 100 Tests/1ml
      CD3 Antibody : Purified 1F4 C, F, IP, P * 0.1 mg | 0.2 mg
      CD3 Antibody : FITC 1F4 F 0.1 mg | 50 µg
      CD3 Antibody : Biotin 1F4 F 0.1 mg
      CD3 Antibody : Alexa Fluor® 700 1F4 F 100 Tests/1ml
      • Copyright © 2016 Bio-Rad

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                • Application NameReference Images
                  Immunohistology - Frozen
                  Immunohistology - Paraffin

                References

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