IgG Antibody

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Published customer image:
RPE conjugated Sheep anti Rabbit IgG antibody used for the detection of bound rabbit anti mPGES-2 and anti cPGES antibodies by flow cytometry.
Image caption:
Effect of TNFa on protein expression of PGE synthases and COX-2 and production of PGE2. (A) Gingival fibroblasts were treated with TNFa (20 ng/ml) or without TNFa for the indicated time period. Cells were harvested, total protein was extracted and protein expression of the PGE2-synthesizing enzymes mPGES-1, mPGES-2, cPGES and COX-2 as well as the loading control actin was analyzed by western blot. (B) Gingival fibroblasts were stimulated with TNFa (20 ng/ml) or cultured without TNFa (control) for 24 h. Protein expression of mPGES-1, mPGES-2, cPGES and COX-2 was investigated by flow cytometry, using specific antibodies. (C) Gingival fibroblasts were cultured with or without TNFa (20 ng/ml) for the indicated time period. Levels of PGE2 in the culture media were measured by EIA on a Luminex system, and are presented as mean ± s.d. Asterisks (*) indicate a significant difference from untreated cells at the corresponding time point (p < 0.05). The results are representative for all three cell lines and all analyses were performed in triplicates.

From: Båge T, Lindberg J, Lundeberg J, Modéer T, Yucel-Lindberg T. Signal pathways JNK and NF-kappaB, identified by global gene expression profiling, are involved in regulation of TNFalpha-induced mPGES-1 and COX-2 expression in gingival fibroblasts. BMC Genomics. 2010 Apr 15;11:241.

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  • Sheep anti Rabbit IgG:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR35AFdatasheet pdfdatasheet pdf1 ml
    STAR35A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Rabbit IgG antibody recognizes rabbit IgG.
    • Intended Use
    • Target Species
      Rabbit
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
    • Antiserum Preparation
      Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Rabbit IgG.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 50ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : RPE F 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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