Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium Falciparum LDH (HCA158) was used as the coating antibody. The antigen (recombinant LDH from Plasmodium falciparum in PBST) was titrated using the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium Falciparum LDH
HCA158 specifically recognises LDH (L-Lactose Dehydrogenase) from the Malaria causing protozoan species Plasmodium falciparum.
Clone 14008 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.
HCA158 is designed for use as a capture antibody with HCA157P as the detection antibody in sandwich assay development.
HCA158 does not cross react with LDH from Plasmodium vivax.
Human IgG1 antibody (kappa light chain) selected from the HuCAL ® phage display library and expressed in a human cell line. This product is supplied as a liquid.
Purified IgG prepared by affinity chromatography on Protein A.
Recombinant LDH fusion protein from Plasmodium falciparum
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline.
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.