L-LACTATE Dehydrogenase Antibody | 14001

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Sandwich assay with electro-chemiluminescence detection using the MSD Sector Imager instrument. Human anti Plasmodium falciparum LDH (HCA158) was used as the coating antibody. The antigen (recombinant LDH from Plasmodium falciparum in PBST) was titrated using  the indicated concentrations. Detection was performed with ECL-labeled Human anti Plasmodium falciparum LDH y

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  • Product Type
    Monoclonal Antibody
  • Clone
    14001
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA157PEdatasheet pdf0.1 mg
    HCA157P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Plasmodium falciparum LDH antibody, clone 14001 specifically recognizes LDH (L-Lactose Dehydrogenase) from the Malaria causing protozoan species Plasmodium falciparum .

      Clone 14001 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.

      When conjugated this clone is suitable for use as a detection antibody with HCA158 as the capture antibody in a sandwich assay.

      This clone is not cross reactive with LDH from Plasmodium vivax. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 71 °C. .
    • Intended Use
    • Target Species
      Protozoan
    • Product Form
      Human IgG1 antibody selected from the HuCAL ® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
    • Immunogen
      Recombinant LDH fusion protein from Plasmodium falciparum
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      L-lactate dehydrogenase activity
      glycolysis
      oxidation-reduction process
    • UniProt
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
      This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with HCA158 as the capture reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional L-LACTATE Dehydrogenase Antibody Formats

    Formats Clone Applications Sizes available
    L-LACTATE Dehydrogenase Antibody : HRP 14001 E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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