Dengue Virus Type 3 NS1 Antigen

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Recombinant Dengue Virus Type 3 NS1 Antigen

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PIP049A E 100 µg
Recombinant dengue virus type 3 NS1 antigen is a purified preparation of the dengue virus serotype 3 (DENV3), non-structural protein 1 (NS1). DENV3 is one of four (DENV1-4) antigenically distinct, closely related viral serotypes belonging to the Flaviviridae family, genus flavivirus. All four serotypes are known to cause viral infection in humans.

Dengue virus is common mosquito-borne infection and a major cause of morbidity in tropical and subtropical regions. There is currently no vaccine to prevent, or effective anti-viral drugs to treat, dengue virus infection. In many cases infection is assymptomatic and the majority of individuals who get ill only suffer the mild, non-specific febrile symptoms characteristic of dengue fever (DF). Only a minority of infections result in severe disease, manifesting as dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Dengue virus infection gives lifelong immunity to the serotype in question but subsequent infection with another serotype may increase the likelihood of severe disease.

The NS1 glycoprotein is essential for viral replication and viability, and since this protein is secreted into the bloodstream, tests have been developed to diagnose DENV infections using NS1, including antigen-capture ELISA, lateral flow antigen detection, and the measurement of NS1-specific IgM and IgG responses (Guzman, M.G. et al. 2010).

Recombinant DENV3 NS1 antigen is presented in its native folded state complete with post-translational modifications, delivering optimal antigenicity and making it suitable for use in vaccine research and serology-based assays.

Product Details

Target Species
Viral
Product Form
Purified recombinant protein with a C-terminal 6 x His-tag - liquid
Preparation
Recombinant dengue virus serotype 3 NS1 protein, sequence strain Sri Lanka D3/H/IMTSSA-SRI/2000/1266, expressed in 293 human cells
Buffer Solution
Dulbecco's phosphate buffered saline
Preservative Stabilisers
None present
Purity
>95% by SDS PAGE analysis
Approx. Protein Concentrations
Approximate protein concentration 0.5 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of Dengue Virus Type 3 NS1 Antigen

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

Further Reading

  1. Guzman, M.G. et al. (2010) Dengue: a continuing global threat.
    Nat Rev Microbiol. 8 (12 Suppl): S7-16.