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Adenovirus

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Native Adenovirus

Product Type
Purified Protein
Specificity
Adenovirus

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0152-0507
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Grade 2 adenovirus, adenoid 6 strain grown in MRC-5 cells in tissue culture. The preparation contains a high concentration of virus and viral components as well as some cellular material in MEM.

Target Species
Viral
Product Form
Semi pure suspension of Adenoid 6 strain, grade 2 - liquid
Preparation
Grown in MRC-5 cells. Optimally infected cells are disrupted by sonication and subjected to low speed centrifugation. The cell lysate is pooled with supernatant from the infected culture and concentrated using ultrafiltration with a 30 kDa cut off.
Buffer Solution
Minimum Essential Media
Preservative Stabilisers
See Buffer solution
Activity
Antigenic activity is 80% of internal reference standard
Approx. Protein Concentrations
Current, batch-specific concentration 2.3 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
The preparation should be sonicated immediately prior to use in order to ensure that the material is uniform
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