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IgE | IR162

Rat IgE Kappa

Product Type
Purified Protein

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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Rat IgE is generated by a spontaneous immunocytoma isolated from a LOU/Wsl rat (Bazin 1974, Bazin & Beckers 1974). The determinant recognized by IgE clone IR162 appears to be shared by several helminthic parasites, including Anisakis simplex, Clonorchis sinensis and Toxocara canis (Cho et al. 2000).

Target Species
Product Form
IgE purified (myeloma IR162) - liquid
Purified IgE prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
Approx. Protein Concentrations
IgE concentration 1.0 mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.1ug/ml 1.25ug/ml
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.

References for IgE

  1. Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
    J. Natl. Cancer Inst. 51: 1359-1361.
  2. Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
    Immunology. 26: 713-723.
  3. Bazin, H. and Beckers, A. (1974) IgE- Myelomas in Rats
    Nobel Symposium 33: Molecular and Biological Aspects of the Acute Allergic Reaction.
  4. Zeuthen, J. et al. (1980) IgE production in vitro by two rat myeloma tumor lines (IR2 and IR162).
    Cell Differ. 9 (3): 137-51.
  5. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J. Immunol. Methods. 71: 9-16.
  6. Bazin, H. J. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    Immunol. Methods. 66: 261-269.
  7. Bazin, H. (Ed) (1990) Rat Hybridomas and Rat Monoclonal Antibodies
    CRC Press, Boca Raton, Florida, USA.
  8. Tulinská J et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
    Arch Toxicol. 92 (7): 2385-99.
  9. View The Latest Product References
  10. Masuda, A. et al. (2018) Body Protein Reserves Sustain Maternal Performance in Early Lactation but Dietary Protein Is Necessary to Maintain Performance and Immune Responses to Nippostrongylus brasiliensis. in Lactating Rats.
    J Nutr. 148 (10): 1638-46.

Entrez Gene
GO Terms
GO:0003823 antigen binding


154191 158754

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