IgE | IR162
Product Details
- Target Species
- Rat
- Product Form
- IgE purified (myeloma IR162) - liquid
- Preparation
- Purified IgE prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Purity
- Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
- Approx. Protein Concentrations
- IgE concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P01855 Related reagents
- Entrez Gene
- Ighe Related reagents
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgE
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.1ug/ml | 1.25ug/ml |
Product Specific References
References for IgE
-
Bazin, H. (Ed) (1990) Rat Hybridomas and Rat Monoclonal Antibodies
CRC Press, Boca Raton, Florida, USA. -
Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
J. Natl. Cancer Inst. 51: 1359-1361. -
Bazin, H. and Beckers, A. (1974) IgE- Myelomas in Rats
Nobel Symposium 33: Molecular and Biological Aspects of the Acute Allergic Reaction. -
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26: 713-723. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J. Immunol. Methods. 71: 9-16. -
Bazin, H. J. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
Immunol. Methods. 66: 261-269. -
Zeuthen, J. et al. (1980) IgE production in vitro by two rat myeloma tumor lines (IR2 and IR162).
Cell Differ. 9 (3): 137-51. -
Tulinská J et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99.