is generated by a spontaneous immunocytoma isolated from a LOU/Wsl rat (Bazin 1974
, Bazin & Beckers 1974
). The determinant recognized by IgE clone IR162 appears to be shared by several helminthic parasites, including Anisakis simplex
, Clonorchis sinensis
and Toxocara canis
(Cho et al. 2000
- Target Species
- Product Form
- IgE purified (myeloma IR162) - liquid
- Purified IgE prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
- Approx. Protein Concentrations
- IgE concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgE
Bazin, H. (Ed) (1990) Rat Hybridomas and Rat Monoclonal Antibodies
CRC Press, Boca Raton, Florida, USA.
Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
J. Natl. Cancer Inst. 51: 1359-1361.
Bazin, H. and Beckers, A. (1974) IgE- Myelomas in Rats
Nobel Symposium 33: Molecular and Biological Aspects of the Acute Allergic Reaction.
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26: 713-723.
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J. Immunol. Methods. 71: 9-16.
Bazin, H. J. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
Immunol. Methods. 66: 261-269.
Zeuthen, J. et al. (1980) IgE production in vitro by two rat myeloma tumor lines (IR2 and IR162).
Cell Differ. 9 (3): 137-51.
Tulinská J et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99.
Masuda, A. et al. (2018) Body Protein Reserves Sustain Maternal Performance in Early Lactation but Dietary Protein Is Necessary to Maintain Performance and Immune Responses to Nippostrongylus brasiliensis. in Lactating Rats.
J Nutr. 148 (10): 1638-46.
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