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IgE | IR162

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Rat IgE Kappa, clone IR162 thumbnail image 1
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Published customer image:
Purified Rat IgE kappa (PRP07A) used for the generation of an IgE concentration standard curve by ELISA.
Image caption:
(A) IgE standard curve. (B) Assessment of the intra/inter-assay variability by reproducing the IgE immunoassay on various plates on the same day or on two separate days (1 week apart) with the selected IgE antibody (CV, coefficient of variation). mAbs414nm, absorbance unit at 414 nm.

From: Tulinská J, Adel-Patient K, Bernard H, Líšková A, Kuricová M, Ilavská S, Horváthová M, Kebis A, Rollerová E, Babincová J, Aláčová R, Wal JM, Schmidt K, Schmidtke J, Schmidt P, Kohl C, Wilhelm R, Schiemann J, Steinberg P. Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days.
(EU 7th Framework Programme project GRACE).
Arch Toxicol. 2018 Jul;92(7):2385-99.
doi: 10.1007/s00204-018-2230-z.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Rat IgE Kappa

Product Type
Purified Protein
Clone
IR162
Isotype
IgE
Specificity
IgE
Region
KAPPA

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PRP07A
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Rat IgE is generated by a spontaneous immunocytoma isolated from a LOU/Wsl rat (Bazin 1974, Bazin & Beckers 1974). The determinant recognized by IgE clone IR162 appears to be shared by several helminthic parasites, including Anisakis simplex, Clonorchis sinensis and Toxocara canis (Cho et al. 2000).

Target Species
Rat
Product Form
IgE purified (myeloma IR162) - liquid
Preparation
Purified IgE prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Purity
Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
Approx. Protein Concentrations
IgE concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.1ug/ml 1.25ug/ml
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.

References for IgE

  1. Bazin, H. (Ed) (1990) Rat Hybridomas and Rat Monoclonal Antibodies
    CRC Press, Boca Raton, Florida, USA.
  2. Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
    J. Natl. Cancer Inst. 51: 1359-1361.
  3. Bazin, H. and Beckers, A. (1974) IgE- Myelomas in Rats
    Nobel Symposium 33: Molecular and Biological Aspects of the Acute Allergic Reaction.
  4. Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
    Immunology. 26: 713-723.
  5. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J. Immunol. Methods. 71: 9-16.
  6. Bazin, H. J. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    Immunol. Methods. 66: 261-269.
  7. Zeuthen, J. et al. (1980) IgE production in vitro by two rat myeloma tumor lines (IR2 and IR162).
    Cell Differ. 9 (3): 137-51.
  8. Tulinská J et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
    Arch Toxicol. 92 (7): 2385-99.
    9. View The Latest Product References
  9. Masuda, A. et al. (2018) Body Protein Reserves Sustain Maternal Performance in Early Lactation but Dietary Protein Is Necessary to Maintain Performance and Immune Responses to Nippostrongylus brasiliensis. in Lactating Rats.
    J Nutr. 148 (10): 1638-46.

RRID
AB_482877
UniProt
P01855
Entrez Gene
Ighe
GO Terms
GO:0003823 antigen binding
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