IgE | IR162
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|Rat IgE is generated by a spontaneous immunocytoma isolated from a LOU/Wsl rat (Bazin 1974, Bazin & Beckers 1974). The determinant recognized by IgE clone IR162 appears to be shared by several helminthic parasites, including Anisakis simplex, Clonorchis sinensis and Toxocara canis (Cho et al. 2000).|
- Target Species
- Product Form
- IgE purified (myeloma IR162) - liquid
- Purified IgE prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide
- Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
- Approx. Protein Concentrations
- IgE concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
References for IgE
Bazin, H. (Ed) (1990) Rat Hybridomas and Rat Monoclonal Antibodies
CRC Press, Boca Raton, Florida, USA.
Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
J. Natl. Cancer Inst. 51: 1359-1361.
Bazin, H. and Beckers, A. (1974) IgE- Myelomas in Rats
Nobel Symposium 33: Molecular and Biological Aspects of the Acute Allergic Reaction.
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26: 713-723.
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J. Immunol. Methods. 71: 9-16.
Bazin, H. J. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
Immunol. Methods. 66: 261-269.
Zeuthen, J. et al. (1980) IgE production in vitro by two rat myeloma tumor lines (IR2 and IR162).
Cell Differ. 9 (3): 137-51.
Tulinská J et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99.
View The Latest Product References
Masuda, A. et al. (2018) Body Protein Reserves Sustain Maternal Performance in Early Lactation but Dietary Protein Is Necessary to Maintain Performance and Immune Responses to Nippostrongylus brasiliensis. in Lactating Rats.
J Nutr. 148 (10): 1638-46.
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