IgE | IR162
Rat IgE Kappa
- Product Type
- Purified Protein
- Clone
- IR162
- Isotype
- IgE
- Specificity
- IgE
- Region
- KAPPA
Rat IgE is generated by a spontaneous immunocytoma isolated from a LOU/Wsl rat (Bazin 1974, Bazin & Beckers 1974). The determinant recognized by IgE clone IR162 appears to be shared by several helminthic parasites, including Anisakis simplex, Clonorchis sinensis and Toxocara canis (Cho et al. 2000). |
- Target Species
- Rat
- Product Form
- IgE purified (myeloma IR162) - liquid
- Preparation
- Purified IgE prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Purity
- Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
- Approx. Protein Concentrations
- IgE concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.1ug/ml | 1.25ug/ml |
References for IgE
-
Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
J. Natl. Cancer Inst. 51: 1359-1361. -
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26: 713-723. -
Bazin, H. and Beckers, A. (1974) IgE- Myelomas in Rats
Nobel Symposium 33: Molecular and Biological Aspects of the Acute Allergic Reaction. -
Zeuthen, J. et al. (1980) IgE production in vitro by two rat myeloma tumor lines (IR2 and IR162).
Cell Differ. 9 (3): 137-51. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J. Immunol. Methods. 71: 9-16. -
Bazin, H. J. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
Immunol. Methods. 66: 261-269. -
Bazin, H. (Ed) (1990) Rat Hybridomas and Rat Monoclonal Antibodies
CRC Press, Boca Raton, Florida, USA. -
Tulinská J et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99.
View The Latest Product References
-
Masuda, A. et al. (2018) Body Protein Reserves Sustain Maternal Performance in Early Lactation but Dietary Protein Is Necessary to Maintain Performance and Immune Responses to Nippostrongylus brasiliensis. in Lactating Rats.
J Nutr. 148 (10): 1638-46.
- RRID
- AB_482877
- UniProt
- P01855
- Entrez Gene
- Ighe
- GO Terms
- GO:0003823 antigen binding
PRP07A
154191 158754If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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