IgE | IR162
Purified Rat IgE kappa (PRP07A) used for the generation of an IgE concentration standard curve by ELISA.
Image caption:
(A) IgE standard curve. (B) Assessment of the intra/inter-assay variability by reproducing the IgE immunoassay on various plates on the same day or on two separate days (1 week apart) with the selected IgE antibody (CV, coefficient of variation). mAbs414nm, absorbance unit at 414 nm.
From: Tulinská J, Adel-Patient K, Bernard H, Líšková A, Kuricová M, Ilavská S, Horváthová M, Kebis A, Rollerová E, Babincová J, Aláčová R, Wal JM, Schmidt K, Schmidtke J, Schmidt P, Kohl C, Wilhelm R, Schiemann J, Steinberg P. Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days.
(EU 7th Framework Programme project GRACE).
Arch Toxicol. 2018 Jul;92(7):2385-99.
doi: 10.1007/s00204-018-2230-z.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat IgE is generated by a spontaneous immunocytoma isolated from a LOU/Wsl rat (Bazin 1974, Bazin & Beckers 1974). The determinant recognized by IgE clone IR162 appears to be shared by several helminthic parasites, including Anisakis simplex, Clonorchis sinensis and Toxocara canis (Cho et al. 2000).
Product Details
- Target Species
- Rat
- Product Form
- IgE purified (myeloma IR162) - liquid
- Preparation
- Purified IgE prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Purity
- Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
- Approx. Protein Concentrations
- IgE concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01855
- Entrez Gene
- Ighe
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgE
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.1ug/ml | 1.25ug/ml |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgE
-
Bazin, H. (Ed) (1990) Rat Hybridomas and Rat Monoclonal Antibodies
CRC Press, Boca Raton, Florida, USA. -
Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
J. Natl. Cancer Inst. 51: 1359-1361. -
Bazin, H. and Beckers, A. (1974) IgE- Myelomas in Rats
Nobel Symposium 33: Molecular and Biological Aspects of the Acute Allergic Reaction. -
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26: 713-723. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J. Immunol. Methods. 71: 9-16. -
Bazin, H. J. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
Immunol. Methods. 66: 261-269. -
Zeuthen, J. et al. (1980) IgE production in vitro by two rat myeloma tumor lines (IR2 and IR162).
Cell Differ. 9 (3): 137-51. -
Tulinská J et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99.
