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IgG

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Purified Rabbit IgG

Product Type
Purified Protein
Isotype
Polyclonal IgG
Specificity
IgG

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Rabbit immunoglobulin G is intended as a negative control reagent for use alongside specific rabbit IgG primary antibodies. It has been tested in flow cytometry on human tissues and demonstrates negligible binding.
Purified Rabbit immunoglobulin G may also be used as a standard for rabbit igG in ELISA (Blakney et al. 2021).

Target Species
Rabbit
Product Form
Purified polyclonal rabbit IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from normal rabbit serum.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 2 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IgG

  1. Khalifeh, M.S. et al. (2010) Investigation of the role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis.
    Lab Anim. 44: 143-9.
  2. Chang, J. et al. (2014) Hypoxia modulates the phenotype of osteoblasts isolated from knee osteoarthritis patients, leading to undermineralized bone nodule formation.
    Arthritis Rheumatol. 66 (7): 1789-99.
  3. Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
    Mol Ther. 29 (3): 1174-85.

RRID
AB_321631
UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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