IgG
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Rabbit immunoglobulin G is intended as a negative control reagent for use alongside specific rabbit IgG primary antibodies. It has been tested in flow cytometry on human tissues and demonstrates negligible binding.
Purified Rabbit immunoglobulin G may also be used as a standard for rabbit igG in ELISA (Blakney et al. 2021).
Purified Rabbit immunoglobulin G may also be used as a standard for rabbit igG in ELISA (Blakney et al. 2021).
Product Details
- Target Species
- Rabbit
- Product Form
- Purified polyclonal rabbit IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified polyclonal rabbit IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from normal rabbit serum
- Preparation
- Purified IgG prepared by ion exchange chromatography from normal rabbit serum.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 2 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01870
- Entrez Gene
- LOC100009097
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | * | ||
| ELISA |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgG
-
Khalifeh, M.S. et al. (2010) Investigation of the role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis.
Lab Anim. 44: 143-9. -
Chang, J. et al. (2014) Hypoxia modulates the phenotype of osteoblasts isolated from knee osteoarthritis patients, leading to undermineralized bone nodule formation.
Arthritis Rheumatol. 66 (7): 1789-99. -
Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
Mol Ther. 29 (3): 1174-85.
Fluorescent Spectraviewer
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