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Purified Rabbit IgG:FITC

Purified Rabbit IgG

Product Type
Purified Protein
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
PRABP01F
Datasheet
F
SDS
0.1 mg loader

PRABP01
Datasheet
E
SDS
10 mg loader

This product is designed as a negative control reagent for use alongside specific rabbit IgG primary antibodies. It has been tested in flow cytometry on human tissues and demonstrates negligible binding.

Product Details

Target Species
Rabbit
Product Form
Purified polyclonal rabbit IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified polyclonal rabbit IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from normal rabbit serum
Preparation
Purified IgG prepared by ion exchange chromatography from normal rabbit serum.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 2 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Product Specific References

References for IgG

  1. Khalifeh, M.S. et al. (2010) Investigation of the role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis.
    Lab Anim. 44: 143-9.
  2. Chang, J. et al. (2014) Hypoxia modulates the phenotype of osteoblasts isolated from knee osteoarthritis patients, leading to undermineralized bone nodule formation.
    Arthritis Rheumatol. 66 (7): 1789-99.
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