Rabbit IgG-FcSPYCATCHER3
Size exclusion chromatography (SEC) was performed on an analytical HPLC instrument. The peak corresponds to monomeric, fully assembled protein.
Column: MAbPac SEC-1.5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, 200 mM arginine, 300 mM NaCl, pH 6.8, flow rate: 200 μl/min; inj. volume: 3 μl; temp.: 25 °C; detection: 280 nm; sample: 1 mg/ml.
Rabbit IgG-FcSpyCatcher3 (rbIgG-FcSpyC3) contains a SpyCatcher3 (Keeble et al. 2019) fused to the hinge region, CH2 and CH3 of rabbit IgG (Hentrich et al. 2020), resulting in a 76.1 kDa protein for the disulfide-bridged dimer. Each SpyCatcher3 subunit can form a stable covalent isopeptide bond with a second protein that includes a SpyTag. The reaction occurs spontaneously on mixing, is rapid, irreversible, high-yielding and shows good specificity.
The reaction is robust at pH 5 to 8, at temperatures from +4°C to +37°C, in various buffer conditions (Ca2+/Mg2+ not needed) and in the presence of detergents. The reaction also occurs inside cells (in vivo). Since the speed of the coupling reaction is concentration dependent, it is recommended to use rbIgG-FcCatcher3 undiluted.
Rabbit IgG-FcSpyCatcher3 is compatible with Bio-Rad's recombinant HuCAL® antibodies with a SpyTag2 at the C-terminus of the heavy chain.
Download FcSpyCatcher coupling protocol
View all available Catchers
Rabbit IgG-FcSpyCatcher3 is compatible with Bio-Rad's recombinant HuCAL® antibodies with a SpyTag2 at the C-terminus of the heavy chain.
Download FcSpyCatcher coupling protocol
View all available Catchers
Product Details
- Target Species
- Protein/peptide tag
- Product Form
- FcSpyCatcher3 purified recombinant protein, fused to the hinge region, CH2, and CH3 of rabbit IgG - liquid
- Preparation
- Recombinant protein expressed in mammalian cell line and purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% ProClin 950
- Purity
- ≥90% determined by SDS-PAGE under reducing conditions and visualized by coomassie blue staining
- Approx. Protein Concentrations
- 80 µM (6.1 mg/ml)
- Protein Molecular Weight
- Predicted 76132 Da
Storage Information
- Storage
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details. - Licensed Use
- For in vitro research purposes. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Rabbit IgG-FcSPYCATCHER3
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoassay |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- View FcSpyCatcher Coupling Protocol
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
Source Reference
-
Zakeri, B. et al. (2012) Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.
Proc Natl Acad Sci U S A. 109 (12): E690-7. -
Keeble, A.H. et al. (2019) Approaching infinite affinity through engineering of peptide-protein interaction.
Proc Natl Acad Sci U S A. Dec 10 [Epub ahead of print].
Antibody Characterization Reference
-
Hentrich, C. et al. (2020) Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly
[Epub ahead of print] [Epub ahead of print]
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