Human IgG3-FcSPYCATCHER3

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Human IgG3-FcSpyCatcher3

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
TZC017
Datasheet
IY
SDS
0.5 mg loader

Human IgG3-FcSpyCatcher3 (hIgG3-FcSpyC3) contains a SpyCatcher3 (Keeble et al. 2019) fused to the hinge region, CH2 and CH3 of human IgG3 (Hentrich et al. 2020), resulting in a 87.4 kDa protein for the disulfide-bridged dimer. Each SpyCatcher3 subunit can form a stable covalent isopeptide bond with a second protein that includes a SpyTag. The reaction occurs spontaneously on mixing, is rapid, irreversible, high-yielding and shows good specificity. The reaction is robust at pH 5 to 8, at temperatures from +4°C to +37°C, in various buffer conditions (Ca2+/Mg2+ not needed) and in the presence of detergents. The reaction also occurs inside cells (in vivo). Since the speed of the coupling reaction is concentration dependent, it is recommended to use hIgG3-FcCatcher3 undiluted.

Human IgG3-FcSpyCatcher3 is compatible with Bio-Rad's recombinant HuCAL® antibodies with a SpyTag2 at the C-terminus of the heavy chain.

Download FcSpyCatcher coupling protocol

View all available Catchers

Product Details

Target Species
Protein/peptide tag
Product Form
Purified recombinant protein - Liquid
Preparation
Recombinant protein expressed in mammalian cell line and purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% ProClin 950
Purity
≥90% determined by SDS-PAGE under reducing conditions and visualized by coomassie blue staining
Approx. Protein Concentrations
80 µM (7 mg/ml)
Protein Molecular Weight
Predicted 87394 Da

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Licensed Use
For in vitro research purposes. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgG3-FcSPYCATCHER3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoassay
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
View FcSpyCatcher Coupling Protocol

Product Specific References

Antibody Characterization Reference

  1. Hentrich, C. et al. (2020) Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly
    [Epub ahead of print]
  2. Zakeri, B. et al. (2012) Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.
    Proc Natl Acad Sci U S A. 109 (12): E690-7.
  3. Keeble, A.H. et al. (2019) Approaching infinite affinity through engineering of peptide-protein interaction.
    Proc Natl Acad Sci U S A. Dec 10 [Epub ahead of print].

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