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VEGF

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Recombinant Mouse VEGF

Product Type
Recombinant Protein
Specificity
VEGF

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Product Code Applications Pack Size List Price Your Price Qty
PMP67
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SDS Safety Datasheet SDS
E FN WB 10 µg loader
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Murine vascular endothelial growth factor (VEGF) is a homodimeric protein, where each subunit is 165 amino acids in length. Murine VEGF is expressed by various vascularized tissues and is reported to stimulate endothelial cell growth and angiogenesis.

Target Species
Mouse
Product Form
Purified Recombinant Protein - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Preparation
Recombinant protein expressed in E. coli
Source
E.coli
Buffer Solution
20mM Acetic Acid
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>98% by SDS page and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
39 kDa (homodimer of 165 amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 1.0ng/ml 5.0ng/ml
Western Blotting 1.5 3.0ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
PMP67 may be used in ELISA applications with either AAM51 or AAM51B.

References for VEGF

  1. Avraham-Lubin, B.C. et al. (2012) VEGF induces neuroglial differentiation in bone marrow-derived stem cells and promotes microglia conversion following mobilization with GM-CSF.
    Stem Cell Rev Rep. 8 (4): 1199-210.

Synonyms
VPF
UniProt
Q00731
Entrez Gene
Vegfa
GO Terms
GO:0005886 plasma membrane
GO:0001541 ovarian follicle development
GO:0001569 patterning of blood vessels
GO:0001701 in utero embryonic development
GO:0001822 kidney development
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003007 heart morphogenesis
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0040007 growth
GO:0007498 mesoderm development
GO:0007595 lactation
GO:0007610 behavior
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0008283 cell proliferation
GO:0030855 epithelial cell differentiation
GO:0031077 post-embryonic camera-type eye development
GO:0042462 eye photoreceptor cell development
GO:0043117 positive regulation of vascular permeability
GO:0043129 surfactant homeostasis
GO:0048010 vascular endothelial growth factor receptor signaling pathway
GO:0048469 cell maturation
GO:0048593 camera-type eye morphogenesis
GO:0048739 cardiac muscle fiber development
GO:0048844 artery morphogenesis
GO:0051781 positive regulation of cell division
GO:0060319 primitive erythrocyte differentiation
GO:0060749 mammary gland alveolus development
GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis

PMP67

155361 159218 166123 166835

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