VEGF
Recombinant Mouse VEGF
- Product Type
- Recombinant Protein
- Specificity
- VEGF
Murine vascular endothelial growth factor (VEGF) is a homodimeric protein, where each subunit is 165 amino acids in length. Murine VEGF is expressed by various vascularized tissues and is reported to stimulate endothelial cell growth and angiogenesis. |
- Target Species
- Mouse
- Product Form
- Purified Recombinant Protein - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Preparation
- Recombinant protein expressed in E. coli
- Source
- E.coli
- Buffer Solution
- 20mM Acetic Acid
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Purity
- >98% by SDS page and HPLC analysis
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution
- Protein Molecular Weight
- 39 kDa (homodimer of 165 amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.2 | 0.4ng/well | |
Functional Assays | 1.0ng/ml | 5.0ng/ml | |
Western Blotting | 1.5 | 3.0ng/lane |
References for VEGF
-
Avraham-Lubin, B.C. et al. (2012) VEGF induces neuroglial differentiation in bone marrow-derived stem cells and promotes microglia conversion following mobilization with GM-CSF.
Stem Cell Rev Rep. 8 (4): 1199-210.
- Synonyms
- VPF
- UniProt
- Q00731
- Entrez Gene
- Vegfa
- GO Terms
- GO:0005886 plasma membrane
- GO:0001541 ovarian follicle development
- GO:0001569 patterning of blood vessels
- GO:0001701 in utero embryonic development
- GO:0001822 kidney development
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0003007 heart morphogenesis
- GO:0005615 extracellular space
- View More GO Terms
- GO:0006916 anti-apoptosis
- GO:0040007 growth
- GO:0007498 mesoderm development
- GO:0007595 lactation
- GO:0007610 behavior
- GO:0008083 growth factor activity
- GO:0008201 heparin binding
- GO:0008283 cell proliferation
- GO:0030855 epithelial cell differentiation
- GO:0031077 post-embryonic camera-type eye development
- GO:0042462 eye photoreceptor cell development
- GO:0043117 positive regulation of vascular permeability
- GO:0043129 surfactant homeostasis
- GO:0048010 vascular endothelial growth factor receptor signaling pathway
- GO:0048469 cell maturation
- GO:0048593 camera-type eye morphogenesis
- GO:0048739 cardiac muscle fiber development
- GO:0048844 artery morphogenesis
- GO:0051781 positive regulation of cell division
- GO:0060319 primitive erythrocyte differentiation
- GO:0060749 mammary gland alveolus development
- GO:0090190 positive regulation of branching involved in ureteric bud morphogenesis
PMP67
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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