M-CSF

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Recombinant Mouse M-CSF

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP80 FN 10 µg
M-CSF (macrophage colony-stimulating factor) is a potent lineage restricted hematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.

Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor). A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodeling.

Product Details

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 100 ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant M-CSF expressed in E.coli
Preservative Stabilisers
None present
Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
Purity
%98 by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml
Protein Molecular Weight
36.4 kDa (156 amino acid homodimer)
Endotoxin Level
<1EU/ug

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution

More Information

UniProt
P07141 Related reagents
Entrez Gene
Csf1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001954 positive regulation of cell-matrix adhesion
GO:0003006 developmental process involved in reproduction
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005157 macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0030278 regulation of ossification
GO:0030335 positive regulation of cell migration
GO:0040018 positive regulation of multicellular organism growth
GO:0042488 positive regulation of odontogenesis of dentine-containing tooth
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
GO:0045651 positive regulation of macrophage differentiation
GO:0045657 positive regulation of monocyte differentiation
GO:0045672 positive regulation of osteoclast differentiation
GO:0045860 positive regulation of protein kinase activity
GO:0046579 positive regulation of Ras protein signal transduction
GO:0048873 homeostasis of number of cells within a tissue
GO:0060444 branching involved in mammary gland duct morphogenesis
GO:0060611 mammary gland fat development
GO:0060763 mammary duct terminal end bud growth
Regulatory
For research purposes only

Applications of M-CSF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.