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IL-7

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Recombinant Mouse Interleukin-7

Product Type
Recombinant Protein
Specificity
IL-7

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Recombinant mouse IL-7 has an ED50 of 0.2ng/ml, determined in a dose response proliferation assay of murine 2E8 cells.

Target Species
Mouse
Product Form
Purified cytokine from E. coli - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water. For extended storage it is recommended to further dilute in a buffer containing 0.1% BSA. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Preparation
Recombinant IL-7 is purified by sequential chromatography
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml
Protein Molecular Weight
15kDa (129 Amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 0.1ng/ml 1ng/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt
P10168
Entrez Gene
Il7
GO Terms
GO:0002360 T cell lineage commitment
GO:0005125 cytokine activity
GO:0005139 interleukin-7 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0010468 regulation of gene expression
GO:0030890 positive regulation of B cell proliferation
GO:0043086 negative regulation of catalytic activity
GO:0045453 bone resorption
GO:0045582 positive regulation of T cell differentiation
GO:0046622 positive regulation of organ growth
GO:0048873 homeostasis of number of cells within a tissue
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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