IL-7

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Recombinant Mouse Interleukin-7

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PMP16A Datasheet	FN SDS	10 µg	Log in		Get Quote

Summary
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Recombinant mouse IL-7 has an ED50 of 0.2ng/ml, determined in a dose response proliferation assay of murine 2E8 cells.

Product Details

Target Species: Mouse
Product Form: Purified cytokine from E. coli - lyophilised
Reconstitution: Reconstitute with 0.1 ml distilled water. For extended storage it is recommended to further dilute in a buffer containing 0.1% BSA. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Preparation: Recombinant IL-7 is purified by sequential chromatography
Preservative Stabilisers: None present
Carrier Free: Yes
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 0.1 mg/ml
Protein Molecular Weight: 15kDa (129 Amino acid sequence)
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P10168
Entrez Gene: Il7
GO Terms: GO:0002360 T cell lineage commitment; GO:0005125 cytokine activity; GO:0005139 interleukin-7 receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0006955 immune response; GO:0008083 growth factor activity; GO:0010468 regulation of gene expression; GO:0030890 positive regulation of B cell proliferation; GO:0043086 negative regulation of catalytic activity; GO:0045453 bone resorption; GO:0045582 positive regulation of T cell differentiation; GO:0046622 positive regulation of organ growth; GO:0048873 homeostasis of number of cells within a tissue
Regulatory: For research purposes only

Applications of IL-7

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Functional Assays		0.1ng/ml	1ng/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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