IL-2

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Recombinant Mouse Interleukin-2

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PMP38
Datasheet
E FN WB
MSDS
20 µg loader

Interleukin-2 (IL-2) is a potent lymphoid cell growth factor which primarily acts on T-cells.

Product Details

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.2ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in E.coli
Buffer Solution
10mM Sodium Citrate pH4.0
Preservative Stabilisers
None present
Activity
5 x 106 Units/mg
Purity
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
0.1 mg/ml after reconstitution
Protein Molecular Weight
17.2 kDa (149 amino acid sequence)
Endotoxin Level
< 0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
P04351 Related reagents
Entrez Gene
Il2 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005134 interleukin-2 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0042104 positive regulation of activated T cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046013 regulation of T cell homeostatic proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050672 negative regulation of lymphocyte proliferation
GO:0050728 negative regulation of inflammatory response
GO:0051024 positive regulation of immunoglobulin secretion
Regulatory
For research purposes only

Applications of IL-2

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4 ng/well
Functional Assays 0.1 1.0 ng/ml
Western Blotting 1.5 3.0 ng/lane
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a standard in ELISA applications either AAM35 or AAM35B.
Western Blotting
This product may be used as the positive control in Western Blotting applications either AAM35 or AAM35B.

Product Specific References

References for IL-2

  1. Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response.
    Proc Natl Acad Sci U S A. 109: 20071-6.