IGF-I

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Recombinant Mouse Insulin-Like Growth Factor I

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PMP44 E FN WB 10 µg
Recombinant Mouse anti insulin-like growth factor I is an E. coli-derived recombinant protein corresponding to full-length mature murine insulin-like growth factor 1 (IGF-1), a 70 amino acid peptide that is structurally and functionally related to insulin but with higher growth-promoting activities.

Recombinant mouse IGF-1 has an ED50 of <2.0ng/ml as determined by a cell proliferation assay using FDC-P1 cells.

Product Details

Target Species
Mouse
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 10 ul distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Preservative Stabilisers
None present
Purity
>97% by SDS PAGE and HPLC
Approx. Protein Concentrations
1.0 mg/ml after reconstitution
Protein Molecular Weight
7.6kD (70 amino acid sequence)
Endotoxin Level
<0.1 ng/ug

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P05017 Related reagents
Entrez Gene
Igf1 Related reagents
GO Terms
GO:0001649 osteoblast differentiation
GO:0001932 regulation of protein phosphorylation
GO:0001974 blood vessel remodeling
GO:0005159 insulin-like growth factor receptor binding
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0008083 growth factor activity
GO:0008285 negative regulation of cell proliferation
GO:0010001 glial cell differentiation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0046326 positive regulation of glucose import
GO:0021940 positive regulation of cerebellar granule cell precursor proliferation
GO:0030104 water homeostasis
GO:0030879 mammary gland development
GO:0031017 exocrine pancreas development
GO:0032878 regulation of establishment or maintenance of cell polarity
GO:0035264 multicellular organism growth
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048286 lung alveolus development
GO:0048468 cell development
GO:0048754 branching morphogenesis of a tube
GO:0050650 chondroitin sulfate proteoglycan biosynthetic process
GO:0051897 positive regulation of protein kinase B signaling cascade
GO:0060426 lung vasculature development
GO:0060463 lung lobe morphogenesis
GO:0060509 Type I pneumocyte differentiation
GO:0060510 Type II pneumocyte differentiation
GO:0060527 prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis
GO:0060736 prostate gland growth
GO:0060741 prostate gland stromal morphogenesis
GO:0060766 negative regulation of androgen receptor signaling pathway
GO:0070373 negative regulation of ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of IGF-I

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 0.1 1.0ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate positive/negative controls.
ELISA
PMP44 may be used as a standard in either an indirect ELISA with AAM30.