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MCP-1

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Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 (MCA2486) as a capture reagent and biotinylated Rabbit anti Human MCP-1 as a detection reagent with recombinant human MCP-1 (PHP061) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are included undiluted.

Recombinant Human MCP-1/Mcaf

Product Type
Recombinant Protein
Specificity
MCP-1

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Product Code Applications Pack Size List Price Your Price Qty
PHP061
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 20 µg loader
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Recombinant human MCP-1/MCAF also known as C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemoattractant protein 1, HC11 or Small-inducible cytokine A2 is encoded by the CCL2 gene. Recombinant MCP-1 is a 76 amino acid ~13kDa chemokine involved in chemotaxis and polarization of the immune response.

Recombinant human MCP-1/MCAF has been employed successfully as a control antigen in the development of a sensitive ELISA employing Mouse anti Human MCP-1 antibody clone 2.2-4A4-1A11 as a capture reagent.

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 0.2 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in E.coli
Source
E.coli
Preservative Stabilisers
None present.
Carrier Free
Yes
Purity
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution.
Protein Molecular Weight
8.6kD (76 Amino acid sequence)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4ng/well
Functional Assays 10ng/ml 100ng/ml
Western Blotting 1.5 3.0ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
ELISA
This product may be used as a standard for ELISA applications with MCA2486.
Western Blotting
This product may be used as the positive control for Western Blotting applications with Mouse anti Human MCP-1 antibody, clone 2.2-4H5-1A11 MCA2486.

Synonyms
CCL2
UniProt
P13500
Entrez Gene
CCL2
GO Terms
GO:0002548 monocyte chemotaxis
GO:0007155 cell adhesion
GO:0004672 protein kinase activity
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006468 protein phosphorylation
GO:0006874 cellular calcium ion homeostasis
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006959 humoral immune response
GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
GO:0007259 JAK-STAT cascade
GO:0008009 chemokine activity
GO:0019079 viral genome replication
GO:0031727 CCR2 chemokine receptor binding
GO:0048246 macrophage chemotaxis

PHP061

156337 156689 164859

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