MCP-1

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Recombinant Human MCP-1/Mcaf

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PHP061 Datasheet	E FN WB SDS	20 µg	Log in		Get Quote

Summary
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Recombinant human MCP-1/MCAF also known as C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemoattractant protein 1, HC11 or Small-inducible cytokine A2 is encoded by the CCL2 gene. Recombinant MCP-1 is a 76 amino acid ~13kDa chemokine involved in chemotaxis and polarization of the immune response.

Recombinant human MCP-1/MCAF has been employed successfully as a control antigen in the development of a sensitive ELISA employing Mouse anti Human MCP-1 antibody clone 2.2-4A4-1A11 as a capture reagent and biotinylated Rabbit anti Human MCP-1 polyclonal antibody (AHP784B) as a detection reagent.

Product Details

Target Species: Human
Product Form: Purified recombinant protein - lyophilised
Reconstitution: Reconstitute with 0.2 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in E.coli
Preservative Stabilisers: None present.
Carrier Free: Yes
Purity: >98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations: 0.1 mg/ml after reconstitution.
Protein Molecular Weight: 8.6kD (76 Amino acid sequence)
Endotoxin Level: < 0.1 ng/ug

Storage Information

Storage: Prior to reconstitution store at -20^oC. Following reconstitution store at -20^oC.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: P13500
Entrez Gene: CCL2
GO Terms: GO:0002548 monocyte chemotaxis; GO:0007155 cell adhesion; GO:0004672 protein kinase activity; GO:0004871 signal transducer activity; GO:0005615 extracellular space; GO:0006468 protein phosphorylation; GO:0006874 cellular calcium ion homeostasis; GO:0006916 anti-apoptosis; GO:0006954 inflammatory response; GO:0006959 humoral immune response; GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger; GO:0007259 JAK-STAT cascade; GO:0008009 chemokine activity; GO:0019079 viral genome replication; GO:0031727 CCR2 chemokine receptor binding; GO:0048246 macrophage chemotaxis
Regulatory: For research purposes only

Applications of MCP-1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.2	0.4ng/well
Functional Assays	10ng/ml	100ng/ml
Western Blotting	1.5	3.0ng/lane

Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.

ELISA: This product may be used as a standard for ELISA applications with AHP784, AHP784B or MCA2486.
Western Blotting: This product may be used as the positive control for Western Blotting applications with AHP784, AHP784B or MCA2486.

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