MCP-1
Recombinant human MCP-1/MCAF also known as C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemoattractant protein 1, HC11 or Small-inducible cytokine A2 is encoded by the CCL2 gene. Recombinant MCP-1 is a 76 amino acid ~13kDa chemokine involved in chemotaxis and polarization of the immune response.
Recombinant human MCP-1/MCAF has been employed successfully as a control antigen in the development of a sensitive ELISA employing Mouse anti Human MCP-1 antibody clone 2.2-4A4-1A11 as a capture reagent and biotinylated Rabbit anti Human MCP-1 polyclonal antibody (AHP784B) as a detection reagent.
Recombinant human MCP-1/MCAF has been employed successfully as a control antigen in the development of a sensitive ELISA employing Mouse anti Human MCP-1 antibody clone 2.2-4A4-1A11 as a capture reagent and biotinylated Rabbit anti Human MCP-1 polyclonal antibody (AHP784B) as a detection reagent.
Product Details
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilised
- Reconstitution
- Reconstitute with 0.2 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in E.coli
- Preservative Stabilisers
- None present.
- Carrier Free
- Yes
- Purity
- >98% by SDS PAGE and HPLC analysis
- Approx. Protein Concentrations
- 0.1 mg/ml after reconstitution.
- Protein Molecular Weight
- 8.6kD (76 Amino acid sequence)
- Endotoxin Level
- < 0.1 ng/ug
Storage Information
- Storage
- Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P13500
- Entrez Gene
- CCL2
- GO Terms
- GO:0002548 monocyte chemotaxis
- GO:0007155 cell adhesion
- GO:0004672 protein kinase activity
- GO:0004871 signal transducer activity
- GO:0005615 extracellular space
- GO:0006468 protein phosphorylation
- GO:0006874 cellular calcium ion homeostasis
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0006959 humoral immune response
- GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
- GO:0007259 JAK-STAT cascade
- GO:0008009 chemokine activity
- GO:0019079 viral genome replication
- GO:0031727 CCR2 chemokine receptor binding
- GO:0048246 macrophage chemotaxis
- Regulatory
- For research purposes only
Applications of MCP-1
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.2 | 0.4ng/well | |
| Functional Assays | 10ng/ml | 100ng/ml | |
| Western Blotting | 1.5 | 3.0ng/lane |
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
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