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Human IgG4 | AbD18705_hIgG4_Pro

Human IgG4 | AbD18705_hIgG4_Pro gallery image 1

SDS-PAGE analysis of recombinant Human IgG4 Kappa (Mutant)

Recombinant Human IgG4 Kappa (Mutant) was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands were visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated. The molecular weight (kDa) of the marker proteins (Lane 1) are displayed on the left hand side of the image

Human IgG4 | AbD18705_hIgG4_Pro gallery image 2

Size exclusion chromatography of recombinant Human IgG4 Kappa (Mutant)

UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG4 Kappa (Mutant). The larger peak corresponds to monomeric, fully assembled AbD18705_hIgG4_Pro. The smaller shoulder most likely contains dimers and aggregates

  • Human IgG4 | AbD18705_hIgG4_Pro thumbnail image 1
  • Human IgG4 | AbD18705_hIgG4_Pro thumbnail image 2
  • Recombinant Human IgG4 Kappa (Mutant)
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  • Product Type
    Recombinant Protein
  • Clone
  • Isotype
    IgG4 Kappa
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA247Edatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant Human IgG4 kappa (mutant), clone AbD18705_hIgG4_Pro is a recombinant human IgG4 antibody with a kappa light chain and a mutation in the core hinge region.

      Clone AbD18705_hIgG4_Pro is specific for green fluorescent protein (GFP) and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG4 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

      In addition to the tetrameric IgG structure, composed of two copies each of the heavy and light chains, wild type human IgG4 forms so-called half-molecules. This species contains only one heavy and one light chain. Half-molecules can assemble with an unrelated half-molecule to form bispecific tetrameric antibody species (Aalberse et al. 2002). Half-molecules of human IgG4 preparations can be detected as an additional species under denaturing, non-reducing conditions using SDS-PAGE, size exclusion chromatography or capillary electrophoresis. Mutation in the core hinge region of IgG4 prevents the formation of half-molecules (Bloom et al. 1997). Recombinant Human IgG4 kappa (mutant) does not display this additional species; half-molecules cannot be detected in SDS-PAGE or by size exclusion chromatography.

      In addition to the mutant recombinant, Bio-Rad also offers recombinant Human IgG4 kappa in its wild type form (HCA195). Recombinant Human IgG4 lambda is also available in both wild type (HCA050) and mutant form (HCA246).
    • Intended Use
    • Product Form
      Human IgG4 antibody selected from the HuCAL® phage display library and expressed in a human cell line - liquid.
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Green fluorescent protein.
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      innate immune response
      antigen binding
      membrane fraction
      complement activation, classical pathway
      protein binding
      immune response
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Human IgG4 Formats

    Formats Clone Applications Sizes available
    Human IgG4 : Purified AbD18705_hIgG4_Pro E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Recombinant Human IgG4 LambdaHCA0500.1 mgE
          Recombinant Human IgG4 KappaHCA1950.1 mgE
          Recombinant Human IgG4 Lambda (Mutant)HCA2460.1 mgE

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Human IgG4 | AbD18705_hIgG4_Pro thumbnail image 1
                • Human IgG4 | AbD18705_hIgG4_Pro thumbnail image 2

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