Human IgG4 | AbD00264_hIgG4

100% Secure


Recombinant Human IgG4 Lambda

Product Type
Recombinant Protein
Clone
AbD00264_hIgG4
Isotype
IgG4 Lambda
Product Code Applications Pack Size List Price Quantity
HCA050A
Datasheet
E
MSDS
50 µg loader

HCA050
Datasheet
E
MSDS
0.1 mg loader

Recombinant Human IgG4 lambda, clone AbD00264_hIgG4 is a recombinant human IgG4 antibody with a lambda light chain.

Clone AbD00264_hIgG4 is specific for green fluorescent protein (GFP) and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG4 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

In addition to the tetrameric IgG structure, composed of two copies each of the heavy and light chains, human IgG4 forms so-called half-molecules. This species contains only one heavy and one light chain. Half-molecules can assemble with an unrelated half-molecule to form bispecific tetrameric antibody species (Aalberse et al. 2002). Half-molecules of human IgG4 preparations can be detected as an additional species under denaturing, non-reducing conditions using SDS-PAGE, size exclusion chromatography or capillary electrophoresis.

Mutation in the core hinge region of IgG4 prevents the formation of half-molecules (Bloom et al. 1997). In addition to the wild type recombinant, Bio-Rad also offers recombinant Human IgG4 lambda in its mutated form (HCA246). Recombinant Human IgG4 kappa is also available in both wild type (HCA195) and mutant form (HCA247).

Product Details

Product Form
Human IgG4 antibody selected from the HuCAL GOLD phage display library and expressed in a human cell line, HKB-11 - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
C6KXN3 Related reagents
P01861 Related reagents
Entrez Gene
IGHG4 Related reagents
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Human IgG4

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 E WB 0.5 ml loader
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader

Product Specific References

Further Reading

  1. Aalberse, R.C. & Schuurman, J. (2002) IgG4 breaking the rules.
    Immunology. 105 (1): 9-19.
  2. Bloom, J.W. et. al. (1997) Intrachain disulfide bond in the core hinge region of human IgG4.
    Protein Sci. 6:407-415