Human IgG2 | AbD00264_hIgG2
Recombinant human IgG2 lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image
UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG2 lambda. The larger peak corresponds to monomeric, fully assembled AbD00264_hIgG2. The smaller shoulder most likely contains dimers and aggregates
Recombinant Human IgG2 lambda contains a lambda light chain. The heavy chain belongs to the G2m(..), previously designated G2m(n-), allotype.
Recombinant Human IgG2 lambda is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.
Recombinant Human IgG2 lambda is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.
Product Details
- Product Form
- Human IgG2 antibody selected from the HuCAL® phage display library and expressed in a human cell line, HKB-11. This antibody is supplied liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Green fluorescent protein
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01859
- Entrez Gene
- IGHG2
- GO Terms
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Human IgG2
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
|
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| Goat anti Human IgG F(ab')2:Alk. Phos. | 0500-0100 | E WB | 0.5 ml |
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| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
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| Goat anti Human IgG F(ab')2 | STAR126 | E | 0.5 mg |
|
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| Goat anti Human IgG F(ab')2:Alk. Phos | STAR126A | E WB | 0.5 ml |
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| Goat anti Human IgG F(ab')2:Biotin | STAR126B | C E F WB | 0.5 mg |
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| Goat anti Human IgG F(ab')2:FITC | STAR126F | C F IF | 0.5 mg |
|
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| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
|
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| Goat anti Human IgG F(ab')2:TRITC | STAR126TRC | C F IF | 0.5 mg |
|
Product Specific References
References for Human IgG2
-
Madan, B. et al. (2016) USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds.
Proc Natl Acad Sci U S A. May 9. pii: 201605691. [Epub ahead of print]
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- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
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- Select combined or multi-laser view to visualize the spectra
