Human IgG2 | AbD00264_hIgG2
Recombinant human IgG2 lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image
UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG2 lambda. The larger peak corresponds to monomeric, fully assembled AbD00264_hIgG2. The smaller shoulder most likely contains dimers and aggregates
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EP SC ResetRecombinant Human IgG2 Lambda
- Product Type
- Recombinant Protein
- Clone
- AbD00264_hIgG2
- Isotype
- IgG2 Lambda
- Specificity
- Human IgG2
- Region
- LAMBDA
Antibody Quality and 
Unrivaled 
| Recombinant Human IgG2 lambda contains a lambda light chain. The heavy chain belongs to the G2m(..), previously designated G2m(n-), allotype. Recombinant Human IgG2 lambda is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent. |
- Product Form
- Human IgG2 antibody selected from the HuCAL phage display library and expressed in a human cell line, HKB-11. This antibody is supplied liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal.
- Immunogen
- Green fluorescent protein
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
References for Human IgG2
-
Madan, B. et al. (2016) USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds.
Proc Natl Acad Sci U S A. May 9. pii: 201605691. [Epub ahead of print]
- RRID
- AB_1963555
- UniProt
- P01859
- Entrez Gene
- IGHG2
- GO Terms
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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