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Human IgG2 | AbD00264_hIgG2

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Recombinant Human IgG2 Lambda, clone AbD00264_hIgG2 thumbnail image 1 Recombinant Human IgG2 Lambda, clone AbD00264_hIgG2 thumbnail image 2
Recombinant Human IgG2 Lambda, clone AbD00264_hIgG2 gallery image 1
SDS-PAGE analysis of recombinant Human IgG2 lambda

Recombinant human IgG2 lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image
Recombinant Human IgG2 Lambda, clone AbD00264_hIgG2 gallery image 2
Size exclusion chromatography of recombinant Human IgG2 lambda

UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG2 lambda. The larger peak corresponds to monomeric, fully assembled AbD00264_hIgG2. The smaller shoulder most likely contains dimers and aggregates

Recombinant Human IgG2 Lambda

Product Type
Recombinant Protein
Clone
AbD00264_hIgG2
Isotype
IgG2 Lambda
Specificity
Human IgG2
Region
LAMBDA

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Product Code Applications Pack Size List Price Your Price Qty
HCA108
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Recombinant Human IgG2 lambda contains a lambda light chain. The heavy chain belongs to the G2m(..), previously designated G2m(n-), allotype.
Recombinant Human IgG2 lambda is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.		 20th Anniversary

Product Form
Human IgG2 antibody selected from the HuCAL phage display library and expressed in a human cell line, HKB-11. This antibody is supplied liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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Description Goat anti Human IgG F(ab')2:HRP
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Goat anti Human IgG F(ab')2:Alk. Phos STAR126A E WB 0.5 ml loader
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Description Goat anti Human IgG F(ab')2:Alk. Phos
Goat anti Human IgG F(ab')2:Biotin STAR126B C E F WB 0.5 mg loader
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Description Goat anti Human IgG F(ab')2:Biotin
Goat anti Human IgG F(ab')2:FITC STAR126F C F IF 0.5 mg loader
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Description Goat anti Human IgG F(ab')2:FITC
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader
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Description Goat anti Human IgG F(ab')2:HRP
Goat anti Human IgG F(ab')2:TRITC STAR126TRC C F IF 0.5 mg loader
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Description Goat anti Human IgG F(ab')2:TRITC

References for Human IgG2

  1. Madan, B. et al. (2016) USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds.
    Proc Natl Acad Sci U S A. May 9. pii: 201605691. [Epub ahead of print]

RRID
AB_1963555
UniProt
P01859
Entrez Gene
IGHG2
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response

HCA108

155000 1607 161119 165782

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