Human IgG2 | AbD00264_hIgG2

Recombinant Human IgG2 Lambda

Product Type
Recombinant Protein
Clone
AbD00264_hIgG2
Isotype
IgG2 Lambda
Specificity
Human IgG2
Region
LAMBDA

Product Code Applications Pack Size List Price Your Price Qty
HCA108
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Recombinant Human IgG2 lambda contains a lambda light chain. The heavy chain belongs to the G2m(..), previously designated G2m(n-), allotype.
Recombinant Human IgG2 lambda is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.

Product Form
Human IgG2 antibody selected from the HuCAL phage display library and expressed in a human cell line, HKB-11. This antibody is supplied liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Green fluorescent protein
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 E WB 0.5 ml loader
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Description Goat anti Human IgG F(ab')2:HRP
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader
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Description Goat anti Human IgG F(ab')2:HRP
Goat anti Human IgG F(ab')2:Alk. Phos. 0500-0100 E WB 0.5 ml loader
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Description Goat anti Human IgG F(ab')2:Alk. Phos.
Goat anti Human IgG F(ab')2 STAR126 E 0.5 mg loader
List Price Your Price
loader
Description Goat anti Human IgG F(ab')2
Goat anti Human IgG F(ab')2:Alk. Phos STAR126A E WB 0.5 ml loader
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loader
Description Goat anti Human IgG F(ab')2:Alk. Phos
Goat anti Human IgG F(ab')2:Biotin STAR126B C E F WB 0.5 mg loader
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loader
Description Goat anti Human IgG F(ab')2:Biotin
Goat anti Human IgG F(ab')2:FITC STAR126F C F IF 0.5 mg loader
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Description Goat anti Human IgG F(ab')2:FITC
Goat anti Human IgG F(ab')2:TRITC STAR126TRC C F IF 0.5 mg loader
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Description Goat anti Human IgG F(ab')2:TRITC
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
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Description Mouse anti Human IgG (Fc) CH2 Domain:HRP

References for Human IgG2

  1. Madan, B. et al. (2016) USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds.
    Proc Natl Acad Sci U S A. May 9. pii: 201605691. [Epub ahead of print]

RRID
AB_1963555
UniProt
P01859
Entrez Gene
IGHG2
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"