IgG

Purified Dog IgG

Product Type
Purified Protein
Specificity
IgG

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PDP004G
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Purified Dog IgG was prepared from normal canine serum by a multi-step process including de-lipidation, salt fractionation and ion exchange chromatography. The product is then extensively dialysed against phosphate buffered saline.
Immunoelectrophoretic analysis demonstrates a single precipitin arc against anti canine IgG and anti canine serum.

Target Species
Dog
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml sterile deionised water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 10.0 mg/ml following recommended reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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