IgG
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Purified Dog IgG was prepared from normal canine serum by a multi-step process including de-lipidation, salt fractionation and ion exchange chromatography. The product is then extensively dialysed against phosphate buffered saline.
Immunoelectrophoretic analysis demonstrates a single precipitin arc against anti canine IgG and anti canine serum.
Immunoelectrophoretic analysis demonstrates a single precipitin arc against anti canine IgG and anti canine serum.
Product Details
- Target Species
- Dog
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile deionised water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 10.0 mg/ml following recommended reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. - Shelf Life
- 12 months from date of reconstitution
More Information
- Regulatory
- For research purposes only
Applications of IgG
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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