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VEGF-A

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Bovine VEGF-A ELISA

Recombinant bovine VEGF-A (PBP027) detected using Rabbit anti Bovine VEGF-A (AHP2382) as the capture reagent and Rabbit anti Bovine VEGF-A:Biotin (AHP2382B) as the detection reagent followed by Streptavidin:HRP

Recombinant Bovine VEGF-A

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PBP027 E 5 µg
Recombinant Bovine VEGF-A, is a recombinant protein corresponding to aa 27 - 190 of native bovine vascular endothelial growth factor A (VEGF-A).

VEGF-A, otherwise known as vascular permeability factor (VPF) is a member of the PDGF / VEGF family of growth factors. Bovine VEGF-A is a growth factor that induces cell proliferation in endothelial cells. It is important in endothelial cell growth, vasculogensis and angiogenesis. VEGF-A is also active in the induction of blood vessel permeability.

Product Details

Target Species
Bovine
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trahalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
19.2 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life
12 months from date of despatch

More Information

UniProt
P15691 Related reagents
Entrez Gene
VEGFA Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0001938 positive regulation of endothelial cell proliferation
GO:0016020 membrane
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0030154 cell differentiation
GO:0042803 protein homodimerization activity
GO:0051781 positive regulation of cell division
GO:0071456 cellular response to hypoxia
Regulatory
For research purposes only

Applications of VEGF-A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2382 as a coating antibody and AHP2382B as a detection antibody.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
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