VEGF-A

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Recombinant Bovine VEGF-A

Product Type: Recombinant Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
PBP027 Datasheet	E SDS	5 µg	Log in		Get Quote

Summary
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Recombinant Bovine VEGF-A, is a recombinant protein corresponding to aa 27 - 190 of native bovine vascular endothelial growth factor A (VEGF-A).

VEGF-A, otherwise known as vascular permeability factor (VPF) is a member of the PDGF / VEGF family of growth factors. Bovine VEGF-A is a growth factor that induces cell proliferation in endothelial cells. It is important in endothelial cell growth, vasculogensis and angiogenesis. VEGF-A is also active in the induction of blood vessel permeability.

Product Details

Target Species: Bovine
Product Form: Purified recombinant protein - lyophilized
Reconstitution: Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 10% Trahalose
Purity: >95% by SDS PAGE analysis
Protein Molecular Weight: 19.2 kDa

Storage Information

Storage: Prior to reconstitution store at -20^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Guarantee: 12 months from date of despatch

More Information

UniProt: P15691
Entrez Gene: VEGFA
GO Terms: GO:0001525 angiogenesis; GO:0001938 positive regulation of endothelial cell proliferation; GO:0016020 membrane; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0008083 growth factor activity; GO:0008201 heparin binding; GO:0030154 cell differentiation; GO:0042803 protein homodimerization activity; GO:0051781 positive regulation of cell division; GO:0071456 cellular response to hypoxia
Regulatory: For research purposes only

Applications of VEGF-A

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2382 as a coating antibody and AHP2382B as a detection antibody.

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