Ubiquitin
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Ubiquitin is a protein modifier that is covalently linked to target lysines and functions in protein degradation.
Product Details
- Target Species
- Bovine
- Product Form
- Purified protein from bovine erythrocytes - lyophilized
- Reconstitution
- Reconstitute with 25 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
- Preservative Stabilisers
- None present
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- 1.0 mg/ml after reconstitution
- Protein Molecular Weight
- 8,500
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P62990
- Regulatory
- For research purposes only
Applications of Ubiquitin
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- 9400-1502 may be used as a standard in an indirect ELISA with 9400-0409G.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Bovine Ubiquitin | 9400-0409G | E | 1 ml |
Product Specific References
References for Ubiquitin
-
Wilkinson, K.D. & Audhya, T.K. (1981) Stimulation of ATP-dependent proteolysis requires ubiquitin with the COOH-terminal sequence Arg-Gly-Gly.
J Biol Chem. 256 (17): 9235-41. -
Levinger, L. & Varshavsky, A. (1982) Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the Drosophila genome.
Cell. 28 (2): 375-85. -
Ciechanover, A. et al. (1984) The ubiquitin-mediated proteolytic pathway and mechanisms of energy-dependent intracellular protein degradation.
J Cell Biochem. 24 (1): 27-53. -
Haas, A.L. & Bright, P.M. (1985) The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates.
J Biol Chem. 260 (23): 12464-73. -
Yang, C.S. et al. (2017) Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9.
Mol Cell. 66 (4): 503-516.e5.
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- Select combined or multi-laser view to visualize the spectra
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