Ubiquitin

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Native Bovine Ubiquitin

Product Type: Purified Protein

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
9400-1502 Datasheet	E SDS	25 mg	Log in		Get Quote

Ubiquitin is a protein modifier that is covalently linked to target lysines and functions in protein degradation.

Product Details

Target Species: Bovine
Product Form: Purified protein from bovine erythrocytes - lyophilized
Reconstitution: Reconstitute with 25 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preservative Stabilisers: None present
Purity: >98% by SDS PAGE
Approx. Protein Concentrations: 1.0 mg/ml after reconstitution
Protein Molecular Weight: 8,500

Storage Information

Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P62990
Regulatory: For research purposes only

Applications of Ubiquitin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

ELISA: 9400-1502 may be used as a standard in an indirect ELISA with 9400-0409G.

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Sheep anti Bovine Ubiquitin	9400-0409G	E	1 ml	Log in		Get Quote

Product Specific References

References for Ubiquitin

Wilkinson, K.D. & Audhya, T.K. (1981) Stimulation of ATP-dependent proteolysis requires ubiquitin with the COOH-terminal sequence Arg-Gly-Gly.
J Biol Chem. 256 (17): 9235-41.
Levinger, L. & Varshavsky, A. (1982) Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the Drosophila genome.
Cell. 28 (2): 375-85.
Ciechanover, A. et al. (1984) The ubiquitin-mediated proteolytic pathway and mechanisms of energy-dependent intracellular protein degradation.
J Cell Biochem. 24 (1): 27-53.
Haas, A.L. & Bright, P.M. (1985) The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates.
J Biol Chem. 260 (23): 12464-73.
Yang, C.S. et al. (2017) Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9.
Mol Cell. 66 (4): 503-516.e5.

Fluorescent Spectraviewer

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How to Use the Spectraviewer?

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Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra