Ubiquitin

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Native Bovine Ubiquitin

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
9400-1502 E 25 mg
Ubiquitin is a protein modifier that is covalently linked to target lysines and functions in protein degradation.

Product Details

Target Species
Bovine
Product Form
Purified protein from bovine erythrocytes - lyophilised
Reconstitution
Reconstitute with 25 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preservative Stabilisers
None present
Purity
SDS PAGE: >98%
Approx. Protein Concentrations
1.0 mg/ml after reconstitution.
Molecular Weight
MW: 8,500

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P62990 Related reagents
Regulatory
For research purposes only

Applications of Ubiquitin

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
9400-1502 may be used as a standard in an indirect ELISA with 9400-0409G.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Bovine Ubiquitin 9400-0409G 1 ml E

Product Specific References

References for Ubiquitin

  1. Wilkinson, K.D. & Audhya, T.K. (1981) Stimulation of ATP-dependent proteolysis requires ubiquitin with the COOH-terminal sequence Arg-Gly-Gly.
    J Biol Chem. 256 (17): 9235-41.
  2. Levinger, L. & Varshavsky, A. (1982) Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the Drosophila genome.
    Cell. 28 (2): 375-85.
  3. Ciechanover, A. et al. (1984) The ubiquitin-mediated proteolytic pathway and mechanisms of energy-dependent intracellular protein degradation.
    J Cell Biochem. 24 (1): 27-53.
  4. Haas, A.L. & Bright, P.M. (1985) The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates.
    J Biol Chem. 260 (23): 12464-73.
  5. Yang, C.S. et al. (2017) Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9.
    Mol Cell. 66 (4): 503-516.e5.
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